犬尿氨酸通路相关物质在大鼠慢性脑低灌注致认知障碍中的作用

发布时间：2018-12-21 19:31

【摘要】：目的观察慢性脑低灌注大鼠海马组织中干扰素-γ、TNF-α、诱导型NO合酶(iNOS)、吲哚胺2,3-双加氧酶(IDO)和犬尿氨酸(KYN)的表达,探讨慢性脑低灌注致认知功能损害大鼠海马组织中KYN通路相关代谢产物的变化特点。方法选择Wistar大鼠30只,随机分为假手术组15只和双侧颈总动脉永久性结扎组(2VO组)15只。制作慢性脑低灌注大鼠模型,Morris水迷宫检测2VO大鼠空间学习与记忆能力变化,免疫组织化学检测大鼠海马CA1区iNOS、干扰素-γ和TNF-α的表达,ELISA法检测IDO及KYN的水平。结果与假手术组比较,2VO组大鼠第3天开始逃避潜伏期明显延长,平台象限游泳距离百分比明显减少,iNOS、干扰素-γ及TNF-α阳性表达,IDO和KYN水平明显升高[(4.73±0.21)ng/ml vs(0.89±0.06)ng/ml和(1.55±0.12)ng/ml vs(0.19±0.02)ng/ml,P0.05]。结论慢性脑灌注大鼠的病理生理过程包含着炎性反应,而炎性反应的发生很可能刺激了IDO和KYN的高表达,KYN和IDO的蓄积有可能参与其导致认知障碍的过程。
[Abstract]:Objective to investigate the expression of interferon- 纬 (IFN- 纬), TNF- 伪, inducible NO synthase (iNOS),) indole 3-dioxygenase (IDO) and canine uremic acid (KYN) in hippocampus of rats with chronic cerebral hypoperfusion. To investigate the changes of KYN pathway related metabolites in hippocampus of rats with cognitive impairment induced by chronic cerebral hypoperfusion. Methods Thirty Wistar rats were randomly divided into sham-operated group (n = 15) and bilateral common carotid artery permanent ligation group (2VO group) (n = 15). Morris water maze was used to detect the changes of spatial learning and memory in rats with chronic cerebral hypoperfusion. The expression of iNOS, interferon 纬 and TNF- 伪 in CA1 region of hippocampus was detected by immunohistochemistry, and the levels of IDO and KYN were detected by ELISA method. Results compared with the sham operation group, the escape latency of 2VO group was significantly prolonged, the percentage of swimming distance in the platform quadrant was significantly decreased, and the positive expression of iNOS, interferon 纬 and TNF- 伪 were observed in the 2VO group on the 3rd day. The levels of IDO and KYN were significantly increased [(4.73 卤0.21) ng/ml vs (0.89 卤0.06) ng/ml and (1.55 卤0.12) ng/ml vs (0.19 卤0.02) ng/ml,P0.05]. Conclusion the pathophysiological process of chronic cerebral perfusion rats includes inflammatory response, which may stimulate the high expression of IDO and KYN, and the accumulation of KYN and IDO may participate in the process of cognitive impairment.
【作者单位】：山西医科大学第二医院神经内科;
【基金】：山西省科技厅青年基金(2009021042-1)
【分类号】：R749.13

【参考文献】