应激对SAMP8小鼠认知功能及情绪的影响及两者机制研究

发布时间：2019-02-28 18:45

【摘要】：目的研究慢性不可预知温和应激(UCMS)对SAMP8小鼠抑郁情绪、学习记忆能力的影响,及UCMS对海马中脑源性神经营养因子(BDNF)及其受体(Trk B)、神经元凋亡相关因子B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)蛋白表达的影响。方法选取健康、7月龄、雄性SAMP8小鼠20只,对其进行随机分组,分为应激组和非应激组,每组各10只。然后对两组小鼠进行为期21天的慢性不可预知应激,应激完成后再进行水迷宫实验及糖水偏好试验。行为学实验完成后,应激组和非应激组各随机选取6只小鼠进行断头取海马,采用蛋白质印迹法(westernblotting)对BDNF及Trk B、Bcl-2、Bax的蛋白表达量进行测定。结果1经过5天的训练后,应激组小鼠寻找到平台的潜伏期明显增长(P0.05);在第6天撤除平台后,应激组小鼠穿越平台次数比非应激组明显减少(P0.05)。2应激后应激组小鼠比非应激组糖水消耗量明显下降(P0.05)。3与非应激组比较,应激组小鼠BDNF及Trk B蛋白表达明显减少,且具有统计学意义(P0.05)。4与非应激组比较,应激组小鼠海马Bcl-2蛋白表达明显减少,Bax蛋白表达明显增多,且具有统计学意义(P0.05)。结论应激使SAMP8小鼠产生抑郁情绪、学习记忆能力下降;应激导致的BDNF及其Trk B、Bcl-2蛋白表达量减少,Bax蛋白量表达增多。
[Abstract]:Objective to study the effects of chronic unpredictable mild stress (UCMS) on depression, learning and memory ability in SAMP8 mice, and the effect of UCMS on brain-derived neurotrophic factor (BDNF) and its receptor (Trk B), in hippocampus. Effect of apoptosis-related factor B lymphocyte tumor-2 gene (Bcl-2) and Bcl-2-associated X protein (Bax) protein expression. Methods Twenty healthy 7-month-old male SAMP8 mice were randomly divided into two groups: stress group (n = 10) and non-stress group (n = 10). Two groups of mice were subjected to chronic unpredictable stress for 21 days. After the stress was completed, the water maze test and sugar-water preference test were performed. After the behavioral experiment was completed, six mice in the stress group and the non-stress group were randomly selected and the hippocampus was decapitated. The protein expression of BDNF, Trk-B, Bcl-2 and Bax were measured by protein blot (westernblotting). Results (1) after 5 days of training, the latency of finding the platform in the stress group increased significantly (P0.05). After removing the platform on the 6th day, the number of crossing the platform in the stress group was significantly lower than that in the non-stress group (P0.05). 2 the consumption of sugar water in the stress group was significantly lower than that in the non-stress group (P0.05). 3Compared with the non-stress group, the frequency of crossing the platform in the stress group decreased significantly (P0.05). (4) compared with non-stress group, the expression of BDNF and Trk B protein in hippocampus of stress group decreased significantly, and the expression of Bax protein increased significantly, compared with non-stress group, the expression of Bcl-2 and Bax protein in hippocampus of stress group was significantly decreased, and the expression of Bax protein was significantly higher than that of non-stress group. And has statistical significance (P0.05). Conclusion stress induced depression and decreased the ability of learning and memory in SAMP8 mice, the expression of Bcl-2 protein and Bcl-2 protein decreased and the expression of Bax protein increased in BDNF and Trk-B induced by stress.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.4

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