远志总皂苷对阿尔茨海默病大鼠突触可塑性的影响
发布时间：2019-05-13 07:16
【摘要】：目的： 1观察远志总皂苷对AD大鼠在体海马CA_1区神经电生理指标长时程增强的影响。 2观察远志总皂苷对AD大鼠海马CA_1区N-甲基-D-天冬氨酸受体2A亚基表达的影响。方法： 雄性Wistar大鼠随机分为对照组、模型组、高剂量远志总皂苷治疗组(37.5 mg/mL)和低剂量远志总皂苷治疗组(12.5 mg/mL)，每组8只，造模方法为腹腔注射D-半乳糖引起大鼠早衰，再用鹅膏蕈氨酸损毁Meynert核诱导大鼠发生AD的病理变化，，测定并比较各组大鼠神经电生理高频刺激（HFS）前后在体海马CA_1区场兴奋性突触后电位（fEPSP）幅度的变化；测定海马CA_1免疫组织化学NR2AIOD值间接测定其表达。 结果： 1、远志总皂苷对AD大鼠在体海马CA_1区神经电生理指标长时程增强的影响：对照组fEPSP的幅度值在HFS 1分钟、30分钟和60分钟分别达到基线水平的203.17±7.47%、178.15±8.11%和164.17±7.03%水平，而模型组fEPSP幅度较对照组显著降低（p0.05），仅分别达到基线水平的173.63±10.81%、127.12±7.38%和102.88±2.36%。低剂量远志总皂苷组fEPSP的幅度百分比值较模型组升高（p0.05），分别达到基线水平的177.67±14.04%、131.83±4.96%和121.17±4.79%，高剂量远志总皂苷组fEPSP的幅度百分比值较模型组显著升高（p0.05），分别达到基线水平的192.00±2.45%、168.00±2.45%和141.75±9.25%，且高剂量远志总皂苷组fEPSP的幅度百分比值较低剂量组升高（p0.05）。 2、远志总皂苷对AD大鼠海马CA_1区突触结构N-甲基-D-天冬氨酸受体2A亚基表达的影响：对照组、AD组、低、高剂量组的NR2A积分光密度值(IOD值)分别为30.12±3.45、11.74±1.69、20.78±2.66、25.86±2.98。与对照组比较，AD模型组IOD值明显降低（p0.05），间接反映了其表达水平降低。与模型组比较，远志总皂苷高、低剂量组的IOD值增高，间接反映其表达水平明显增高（p0.05, p0.05），并且高剂量组NR2A的表达较低剂量组显著（p0.05）。 结论: 1、远志总皂苷对AD大鼠海马CA_1区神经电生理指标LTP具有易化作用。 2、远志总皂苷可上调AD大鼠海马CA_1区突触结构N-甲基-D-天冬氨酸受体2A亚基表达。 3、远志总皂苷对上述两作用呈现剂量依赖性。
[Abstract]:Aim: 1 to observe the effect of total saponins of Polygaloside on long-term potentiation of neuroelectrophysiological indexes in CA _ 1 region of hippocampus in AD rats. 2 to observe the effect of total saponins of Polygaloside on the expression of N-methyl-D-aspartic acid receptor 2A subunit in hippocampal CA_ 1 region of AD rats. Methods: male Wistar rats were randomly divided into control group, model group, high dose Polygaloside treatment group (37.5 mg/mL) and low dose Polygala saponins treatment group (12.5 mg/mL), with 8 rats in each group. The model was induced by intraperitoneal injection of D-galactose to induce premature senility in rats, and the pathological changes of AD were induced by the destruction of Meynert nucleus by gossypol fungofunc acid. The changes of (fEPSP) amplitude of excitatory postsynaptic potential in hippocampal CA_1 region in vivo before and after high frequency neuroelectrophysiological stimulation of (HFS) were measured and compared. The expression of CA_1 in hippocampus was measured indirectly by NR2AIOD. Results: 1. The effect of total saponins of Polygala tenuifolia on the long-term potentiation of neuroelectrophysiological indexes in hippocampal CA _ 1 region of AD rats: the amplitude of fEPSP in the control group reached 203.17 卤7.47% of the baseline level at 1 minute, 30 minutes and 60 minutes, respectively. The levels of fEPSP in the model group were significantly lower than those in the control group (p0.05), only 173.63 卤10.81%, 127.12 卤7.38% and 102.88 卤2.36%, respectively. The percentage value of fEPSP in the low-dose total saponin group was higher than that in the model group (p0.05), reaching 177.67 卤14.04%, 131.83 卤4.96% and 121.17 卤4.79% of the baseline level, respectively. The percentage value of fEPSP in the high-dose total saponins group was significantly higher than that in the model group (p0.05), reaching 192.00 卤2.45% of the baseline level, 192.00 卤2.45% and 141.75 卤9.25% of the baseline level, respectively. The amplitude percentage ratio of fEPSP in high dose Polygala tenuifolia group was higher than that in low dose group (p0.05). 2. Effects of total saponins of Polygaloside on the expression of N-methyl-D-aspartate receptor 2A subunit in hippocampal CA_ 1 region of AD rats: control group, AD group, low, low. The NR2A integral optical density (IOD) of high dose group was 30.12 卤3.45,11.74 卤1.69, 20.78 卤2.66, 25.86 卤2.98, respectively. Compared with the control group, the IOD value of AD model group was significantly lower (p0.05), which indirectly reflected the decrease of IOD expression level. Compared with the model group, the Iod value of total saponins in high dose group and low dose group was significantly higher than that in low dose group (p0.05, p0.05), and the expression of NR2A in high dose group was significantly higher than that in low dose group (p0.05). Conclusion: 1. Polygala tenuifolia saponins can facilitate the neuroelectrophysiological index LTP in hippocampal CA_1 region of AD rats. 2. Polygaloside could up-regulate the expression of N-methyl-D-aspartate receptor 2A subunit in the synaptic structure of hippocampal CA_ 1 region of AD rats. 3. The total saponins of Polygala tenuifolia showed a dose-dependent effect on the above two effects.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R749.16
本文编号：2475696
[Abstract]:Aim: 1 to observe the effect of total saponins of Polygaloside on long-term potentiation of neuroelectrophysiological indexes in CA _ 1 region of hippocampus in AD rats. 2 to observe the effect of total saponins of Polygaloside on the expression of N-methyl-D-aspartic acid receptor 2A subunit in hippocampal CA_ 1 region of AD rats. Methods: male Wistar rats were randomly divided into control group, model group, high dose Polygaloside treatment group (37.5 mg/mL) and low dose Polygala saponins treatment group (12.5 mg/mL), with 8 rats in each group. The model was induced by intraperitoneal injection of D-galactose to induce premature senility in rats, and the pathological changes of AD were induced by the destruction of Meynert nucleus by gossypol fungofunc acid. The changes of (fEPSP) amplitude of excitatory postsynaptic potential in hippocampal CA_1 region in vivo before and after high frequency neuroelectrophysiological stimulation of (HFS) were measured and compared. The expression of CA_1 in hippocampus was measured indirectly by NR2AIOD. Results: 1. The effect of total saponins of Polygala tenuifolia on the long-term potentiation of neuroelectrophysiological indexes in hippocampal CA _ 1 region of AD rats: the amplitude of fEPSP in the control group reached 203.17 卤7.47% of the baseline level at 1 minute, 30 minutes and 60 minutes, respectively. The levels of fEPSP in the model group were significantly lower than those in the control group (p0.05), only 173.63 卤10.81%, 127.12 卤7.38% and 102.88 卤2.36%, respectively. The percentage value of fEPSP in the low-dose total saponin group was higher than that in the model group (p0.05), reaching 177.67 卤14.04%, 131.83 卤4.96% and 121.17 卤4.79% of the baseline level, respectively. The percentage value of fEPSP in the high-dose total saponins group was significantly higher than that in the model group (p0.05), reaching 192.00 卤2.45% of the baseline level, 192.00 卤2.45% and 141.75 卤9.25% of the baseline level, respectively. The amplitude percentage ratio of fEPSP in high dose Polygala tenuifolia group was higher than that in low dose group (p0.05). 2. Effects of total saponins of Polygaloside on the expression of N-methyl-D-aspartate receptor 2A subunit in hippocampal CA_ 1 region of AD rats: control group, AD group, low, low. The NR2A integral optical density (IOD) of high dose group was 30.12 卤3.45,11.74 卤1.69, 20.78 卤2.66, 25.86 卤2.98, respectively. Compared with the control group, the IOD value of AD model group was significantly lower (p0.05), which indirectly reflected the decrease of IOD expression level. Compared with the model group, the Iod value of total saponins in high dose group and low dose group was significantly higher than that in low dose group (p0.05, p0.05), and the expression of NR2A in high dose group was significantly higher than that in low dose group (p0.05). Conclusion: 1. Polygala tenuifolia saponins can facilitate the neuroelectrophysiological index LTP in hippocampal CA_1 region of AD rats. 2. Polygaloside could up-regulate the expression of N-methyl-D-aspartate receptor 2A subunit in the synaptic structure of hippocampal CA_ 1 region of AD rats. 3. The total saponins of Polygala tenuifolia showed a dose-dependent effect on the above two effects.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R749.16
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