抑郁大鼠海马微管相关蛋白pMAP-2和p-Tau表达的研究

发布时间：2019-06-18 14:37

【摘要】：目的:观察微管蛋白p MAP-2和p-Tau在抑郁大鼠海马的表达变化情况,探讨海马微管蛋白与抑郁症的关系和意义。方法:通过利用慢性不可预见性温和应激(chronic unpredicted mild stress,CUMS)建立大鼠抑郁症模型,对照组和模型组分别采用糖水偏好实验、旷场实验以及Morris水迷宫对其行为进行学检测,采用尼氏染色观察海马神经元的形态学变化,对照组和模型组造模后1、7、14、28 d分批处死大鼠,用Western Blot方法检测海马组织中p-MAP-2和p-Tau的蛋白表达水平,免疫荧光双标观察海马组织中p-MAP-2和p-Tau定位分布。结果:糖水偏好实验:模型组糖水消耗量和糖水偏好百分比分别低于对照组(P0.01);旷场实验:模型组行走总路程、中央活动时间、直立次数和修饰行为分别低于对照组(P0.01);Morris水迷宫模型组平均逃避潜伏期高于对照组(P0.01)。CUMS后1 d大鼠海马p-MAP-2和p-Tau蛋白的表达低于对照组,7 d后开始升高,14 d明显高于对照组,28 d仍略高于对照组。p-MAP-2和p-Tau免疫荧光双标结果显示:部分神经元的胞浆内p-MAP-2和pTau同时表达,表明p-MAP-2和p-Tau有部分共定位。结论:p-MAP-2和p-Tau在抑郁模型大鼠海马出现先降低后增高的现象,因此我们推测在应激抑郁症发病过程中,p-MAP-2和p-Tau蛋白表达的变化可能影响微管的稳定性,破坏细胞骨架稳态,从而影响海马神经元的结构改变。
[Abstract]:Aim: to observe the expression of tubulin p MAP-2 and p-Tau in hippocampus of depressed rats and to explore the relationship between tubulin and depression. Methods: the depression model of rats was established by chronic unpredictable mild stress (chronic unpredicted mild stress,CUMS. The behavior of the control group and the model group were detected by sugar water preference test, open field test and Morris water maze, respectively. the morphological changes of hippocampal neurons were observed by Nissl staining. The rats in the control group and the model group were killed in batches 1, 7, 14 and 28 days after modeling. The protein expression of p-MAP-2 and p-Tau in hippocampal tissue was detected by Western Blot, and the localization and distribution of p-MAP-2 and p-Tau in hippocampal tissue were observed by immunofluorescence double labeling. Results: the sugar water preference experiment: the sugar water consumption and the sugar water preference percentage in the model group were lower than those in the control group (P 0.01), and the open field experiment: the total walking distance, central activity time, upright times and modification behavior in the model group were lower than those in the control group (P 0.01). The average escape latency of Morris water maze model group was higher than that of the control group (P 0.01). The expression of p-MAP-2 and p-Tau protein in hippocampus of rats on the 1st day after CUMS was lower than that of the control group, began to increase after 7 days, was significantly higher than that of the control group on the 14th day, and was still slightly higher than that of the control group on the 28th day. The results of p-MAP-2 and p-Tau immunofluorescence double labeling showed that the expression of p-MAP-2 and pTau in the cytoplasm of some neurons was at the same time. It is shown that p-MAP-2 and p-Tau have partial co-localization. Conclusion: p-MAP-2 and p-Tau decrease at first and then increase in hippocampus of depression model rats. Therefore, we speculate that the changes of p-MAP-2 and p-Tau protein expression may affect the stability of microtubules and destroy the homeostasis of cytoskeleton in the pathogenesis of stress depression, thus affecting the structural changes of hippocampal neurons.
【作者单位】：河北联合大学基础医学院组织胚胎学系;山东万杰医学院医学系;河北联合大学附属医院神经内科;
【基金】：国家自然科学基金(81201048) 河北省自然科学基金(H2012401009)
【分类号】：R749.4

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