孤独症儿童血清鞘脂类代谢产物水平的对照研究

发布时间：2019-07-27 06:35

【摘要】：目的探讨孤独症患儿血清中鞘脂类代谢产物水平及其与临床诊断量表的相关性,评价其对孤独症的潜在诊断价值。方法对73例孤独症儿童和63例正常对照儿童血清鞘脂类进行分析,找出与孤独症有关的代谢产物并测其水平;用同法对另100例孤独症儿童和100例正常对照儿童进行验证,并分析其与孤独症儿童行为检查量表(Autism Behavior Checklist,ABC)和儿童孤独症评定量表(Childhood Autism Rating Scale,CARS)的相关性,绘制ROC曲线。结果筛选出两种与孤独症有关的鞘脂类代谢产物植物鞘氨醇(phytosphingosine,PSO)和1-磷酸鞘氨醇(sphingosine-1-phosphate,S1P),其含量在孤独症组明显升高(P0.05);验证发现孤独症组PSO和S1P含量明显升高(P0.05),S1P与ABC总分呈显著正相关。PSO的ROC曲线得到其曲线下面积(AUC)和Youden指数最大值均为1.000,最佳临界值为44.883,对应的敏感度和特异度为100%。S1P的AUC为0.961,Youden指数最大值为0.868,最佳临界值为47.678,对应的敏感度为91.7%,特异度为95.1%。结论孤独症患儿血清中PSO和S1P含量与正常儿童存在明显差异,提示鞘脂类代谢异常可能与孤独症有一定关联,且二者对孤独症有较好的诊断价值,ROC曲线确定的最佳临界值分别为44.883和47.678。
[Abstract]:Objective to investigate the level of serum sheath lipids metabolites in autistic children and its correlation with clinical diagnostic scale, and to evaluate its potential diagnostic value in autistic children. Methods the serum sheath lipids of 73 autistic children and 63 normal children were analyzed, the metabolites related to autism were found out and their levels were measured, the other 100 autistic children and 100 normal control children were verified by the same method, and the correlation with autistic children behavior scale (Autism Behavior Checklist,ABC) and child autism rating scale (Childhood Autism Rating Scale,CARS) was analyzed, and the ROC curve was drawn. Results two kinds of spannin metabolites, phytosphingosine,PSO and sphingosine-1-phosphate,S1P, were screened out and their contents were significantly increased in autistic group (P 0.05), and their contents were significantly higher than those in autistic group (P 0.05), and their contents were significantly higher than those in autistic group (P 0.05), and their contents were significantly higher than those in autistic group (P 0.05). The results showed that the contents of PSO and S1P in autistic group were significantly increased (P 0.05), and there was a significant positive correlation between S1P and the total score of ABC. The ROC curve showed that the maximum area (AUC) and Youden index under the curve were 1.000, the best critical value was 44.883, the corresponding sensitivity and specificity was 0.961 AUC, the maximum Youden index was 0.868, the best critical value was 47.678, and the corresponding sensitivity was 91.7%. The specificity was 95.1%. Conclusion the contents of PSO and S1P in serum of autistic children are significantly different from those of normal children, suggesting that abnormal sheath lipid metabolism may be related to autism, and they are of good diagnostic value for autism. The best critical values determined by ROC curve are 44.883 and 47.678, respectively.
【作者单位】：哈尔滨医科大学公共卫生学院儿少卫生与妇幼保健学教研室;
【基金】：国家自然科学基金(81302443)
【分类号】：R749.94

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