乳牙根尖周病损组织细菌组成与临床症状的关系

发布时间：2018-01-02 11:19

本文关键词：乳牙根尖周病损组织细菌组成与临床症状的关系　出处：《口腔医学研究》2015年12期 　论文类型：期刊论文

【摘要】：目的:检测乳牙根尖周病损组织中菌群构成,分析检出细菌种类与临床症状的关系。方法:收集31例乳牙根尖周炎患儿的根尖周病损组织,采用16SrDNA克隆测序法检测样本中菌群构成,统计分析菌种检出率与有无自发疼、咀嚼疼及窦道的关系。结果:31个乳牙根尖周病损组织样本共获得2057个可分析序列,鉴定出268个菌种或基因型,从属于9个菌门。检出率最高的具核梭杆菌(74.19%)、隐蔽小杆菌(67.74%)和纤细弯曲杆菌(64.52%)与各临床症状间无明显关联,普氏坦纳菌在有自发痛样本中检出率显著增高,口普氏菌和副溶血嗜血杆菌在有咀嚼痛样本中的检出率显著增高,变黑普氏菌和短优杆菌存在的样本发生窦道的几率显著增高,两种未培养韦荣氏菌的检出率在有咀嚼痛的样本中显著减少。结论:乳牙慢性根尖周病损是包含多种细菌的混合感染,普氏菌、优杆菌及韦荣氏菌与临床症状有一定的关系。
[Abstract]:Objective: to detect bacteria in deciduous periapical lesion tissues, analysis of relation between detection of bacterial species and clinical symptoms. Methods: periapical lesion tissue was collected from 31 cases of breast periapicalitis were cloned by 16SrDNA, sequenced samples of bacteria, bacteria detection rate and there is no spontaneous pain related statistical analysis. Chewing pain and sinus. Results: 31 primary teeth with periapical lesion tissue samples were obtained 2057 available sequence analysis identified 268 strains or genotypes from 9 strains belonging to the door. The highest detection rate of Fusobacterium nucleatum (74.19%), (67.74%) small hidden coli and slender Campylobacter (64.52%) no significant correlation with the clinical symptoms, Platts forsythensis in spontaneous pain in the sample detection rate increased significantly, and Prevotella and Haemophilus parahaemolyticus in chewing pain detection rate was significantly higher in the sample, are black and short bacilli and Prevotella The probability of occurrence of sinus samples significantly, two kinds of uncultured Veillonella were detected in a sample of chewing pain decreased significantly. Conclusion: primary teeth with chronic periapical lesion is mixed infection, contains a variety of bacteria Prevotella, Eubacterium and Veillonella have certain relations with the clinical symptoms.

【作者单位】：首都医科大学口腔医学院儿童口腔科;首都医科大学医学实验与检测中心;
【基金】：北京市自然科学基金资助项目(编号:7122079) 北京市教育委员会科技计划面上项目(编号:SQKM201210025019)
【分类号】：R780.2
【正文快照】： 乳牙根尖周炎常由牙髓感染发展而来,由于乳牙独特的生理解剖特点,根管口粗大、牙髓组织疏松、血运丰富、髓室底侧副根管多,感染极易扩散到根尖周组织,出现疼痛、咀嚼不适等症状。儿童牙周组织和牙槽骨质疏松,乳牙根尖周炎较恒牙更易引起软组织肿胀或形成瘘道。以往研究表明,乳

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