不同粒径羟基磷灰石对人工早期龋再矿化作用的研究

发布时间：2018-01-03 00:23

本文关键词：不同粒径羟基磷灰石对人工早期龋再矿化作用的研究　出处：《郑州大学学报(医学版)》2015年06期 　论文类型：期刊论文

【摘要】：目的:研究不同粒径羟基磷灰石(HA)对人工早期龋的再矿化作用。方法:应用离体人恒前磨牙制备早期釉质龋标本50个,随机分为5组(n=10):阳性对照组(Na F溶液)、阴性对照组(去离子水)、30 nm HA组、60 nm HA组和12μm HA组,用显微硬度计检测标本脱矿前后表面显微硬度,并用扫描电镜观察各组样本脱矿前后的形态;按照分组进行早期龋体外循环实验12 d,同法测定体外循环后各组样本的表面显微硬度,并观察其形态。结果:与阴性对照组相比,各HA组均能提高标本的表面显微硬度,且随着粒径的减小,其表面显微硬度增大(F=86.272,P0.001)。扫描电镜下观察,30 nm HA组和60 nm HA组的脱矿区有针状或短棒状晶体颗粒沉积,表面变得均匀、平整,且以30 nm HA组的再矿化效果更佳,12μm HA组的再矿化效果不明显。结论:HA能有效促进早期釉质龋的再矿化;纳米级HA优于微米级HA,且随着粒径的减小,其再矿化效果更佳。
[Abstract]:Objective: to study the effect of hydroxyapatite (HA) with different particle sizes on the remineralization of artificial early caries. Methods: 50 specimens of early enamel caries were prepared from human permanent premolars in vitro. Five groups were randomly divided into 5 groups: the positive control group (NaF solution), the negative control group (deionized water + 30 nm HA group), the 60 nm HA group and the 12 渭 m HA group. The surface microhardness of the samples before and after demineralization was examined by microhardness meter, and the morphology of each group of samples before and after demineralization was observed by scanning electron microscope. The surface microhardness of each group after CPB was determined by the same method for 12 days. Results: compared with the negative control group, the microhardness of each group was observed. The surface microhardness of each HA group was increased with the decrease of particle size, and the surface microhardness of HA group increased with the decrease of particle size. The microhardness was observed by scanning electron microscope (SEM). The demineralization of 30 nm HA group and 60 nm HA group had needle-shaped or short rod crystal particles deposition, the surface became uniform and smooth, and the 30 nm HA group had better effect of remineralization. The effect of 12 渭 m HA on the remineralization was not obvious. Conclusion the remineralization of early enamel caries can be promoted effectively by the concentration of 12 渭 m HA. Nanometer HA is superior to micron HA, and with the decrease of particle size, the effect of remineralization is better.
【作者单位】：南方医科大学附属口腔医院(广东省口腔医院);南方医科大学珠江医院口腔科;
【基金】：广东省自然科学基金资助项目2014A030313330
【分类号】：R781.1
【正文快照】： 13(10):1193[6]卿松,李晓红,苗娜,等.良、恶性卵巢甲状腺肿临床病理分析[J].中国癌症杂志,2013,23(7):524[7]张伶,关键,李梅,等.卵巢甲状腺肿的CT诊断及病理对照(附3例报道及文献复习)[J].影像诊断与介入放射学,2011,20(5):330[8]Huh JJ,Montz FJ,Btistow RE.Struma ovarii as

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