腺泡细胞在大鼠腮腺组织萎缩过程中的变化规律

发布时间：2018-01-03 20:02

本文关键词：腺泡细胞在大鼠腮腺组织萎缩过程中的变化规律　出处：《实用口腔医学杂志》2015年05期 　论文类型：期刊论文

【摘要】：目的:研究大鼠腮腺主导管结扎诱导腺体萎缩过程中腺泡细胞的变化规律。方法:通过对大鼠腮腺主导管结扎,建立腮腺组织萎缩模型,分别于结扎术后0、1、3、5、7、10、14、21和30 d获取腮腺组织标本,苏木精-伊红(HE)和阿新蓝-过碘酸雪夫(AB-PAS)染色观察腺体的组织学变化,免疫组织化学法检测半胱氨酸天冬氨酸蛋白水解酶3(caspase3)、增殖细胞核抗原(Ki-67)、钙调节蛋白(calponin)和淀粉酶(amylase)的表达变化。结果:组织学观察见导管结扎组大鼠腮腺大部分腺泡细胞萎缩、消失,酶原颗粒消失,仅在腺小叶的边缘残存少量腺泡细胞。Caspase3在结扎7 d组达峰值;Ki-67在结扎3 d组达峰值;各实验组间caspase3及Ki-67表达水平差异均有统计学意义(P0.05)。结论:大鼠腮腺主导管结扎至30 d时,大部分腺泡细胞萎缩、凋亡,分泌功能丧失,但在腺小叶的边缘仍存在少量腺泡细胞。
[Abstract]:Objective: to study the changes of acinar cells in rat parotid gland atrophy induced by ligation of main parotid duct. Methods: the model of parotid gland atrophy was established by ligating the main duct of parotid gland in rats. The parotid tissue specimens were obtained on the 21st and 30th day. The histopathological changes of glands were observed by hematoxylin-eosin (HEH) and acinolan-periodate (AB-PAS) staining. Cysteine aspartate proteolytic enzyme (Caspase-3) and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemistry. The expression of calponin and amylase). Results: histological observation showed that most of the acinar cells of the parotid gland in the group of duct ligation were atrophied and disappeared. The proenzyme granules disappeared and only a few acinar cells. Caspase3 reached the peak value at the 7th day after ligation. The peak value of Ki-67 was reached at 3 days after ligation. The expression of caspase3 and Ki-67 were significantly different among the experimental groups (P 0.05). Conclusion: most of the acinar cells atrophy after ligation of the main parotid duct for 30 days. Apoptosis, loss of secretory function, but there are still a small number of acinar cells at the edge of the lobule.
【作者单位】：吉林大学口腔医院口腔颌面外科;
【基金】：吉林省高技术产业项目(编号:2014N152) 吉林省直厅局项目(编号:5139073431) 吉林大学研究生创新基金资助项目(编号:2015104)
【分类号】：R781
【正文快照】： 唾液腺组织萎缩是口腔颌面外科的常见病、多发病,肿瘤、涎石、放化疗等都会导致腺体的萎缩,造成面部软组织塌陷及唾液分泌功能障碍。因此,在临床治疗上如何防治腺体进行性萎缩、恢复唾液分泌功能,以减轻患者的生理和心理负担成为亟待解决的难题。经典的腮腺浅叶肿瘤切除术中常

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