二肽肽酶Ⅰ对脂多糖诱导的小鼠牙周炎的影响

发布时间：2018-01-05 02:11

本文关键词：二肽肽酶Ⅰ对脂多糖诱导的小鼠牙周炎的影响　出处：《实用口腔医学杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的:探究二肽肽酶I缺失(DPPI)对小鼠发生牙周炎时牙槽骨破坏以及牙周炎症反应的影响。方法:用SPF级C57BL/6J野生型小鼠(WT)10只和同种系DPPI基因敲除小鼠(DPPI-/-)10只,E.coli LPS注射法对2组小鼠左侧上下颌第一磨牙建立牙周炎模型,对右侧上下颌第一磨牙用PBS注射法为对照。建模4周后进行micro CT扫描分析和牙体牙周组织联合切片,HE染色进行组织学观察牙周组织,并比较2组小鼠牙槽骨破坏程度以及牙周炎症反应差异。结果:2组小鼠实验侧相比对照侧牙槽骨均发生了破坏,但DPPI-/-组牙槽骨高度的降低量小于WT组[上颌:(0.001 7±0.000 4)mm vs(0.202 0±0.008 6)mm;下颌:(0.034 2±0.002 9)mm vs(0.332 8±0.012 5 mm)](P0.05)。组织学观察显示WT组小鼠的牙槽骨破坏以及炎细胞浸润程度均大于DPPI-/-组小鼠。结论:DPPI对小鼠牙周炎的发生具有促进作用。
[Abstract]:Objective: to investigate the effects of dipeptidyl peptidase I deletion (DPPI) on alveolar bone destruction and periodontal inflammation in mice with periodontitis. Methods: SPF C57BL / 6J wild-type mice were used to investigate the effects of DPPI on alveolar bone destruction and periodontal inflammation in mice with periodontitis. Ten homologous DPPI gene knockout mice (DPPI-P / P) were used in this study. Coli LPS injection method was used to establish periodontitis model of left maxillary first molar in two groups. The right maxillary first molars were treated with PBS injection. After 4 weeks of modeling, the periodontal tissues were analyzed by micro CT scanning and stained with HE staining of periodontal tissue. The periodontal tissues were observed histologically. The degree of alveolar bone destruction and periodontal inflammation were compared between the two groups. Results the alveolar bone in the experimental side of the two groups was damaged compared with the control group. But the reduction of alveolar bone height in DPPI-P-group was less than that in WT group. [Maxillary: 0. 001 7 卤0. 0004 mm vs(0.202 0 卤0. 008 6 mm; 0. 034 2 卤0. 0029 mm vs(0.332 8 卤0. 012 5 mm). Histological observation showed that the degree of alveolar bone destruction and infiltration of inflammatory cells in WT group was higher than that in DPPI-P-group. Conclusion:% DPPI can promote the occurrence of periodontitis in mice.
【作者单位】：第四军医大学口腔医院牙周病科·军事口腔医学国家重点实验室;第四军医大学口腔医院口腔黏膜病科·军事口腔医学国家重点实验室;
【基金】：国家自然科学基金(编号:81371154,81271137)
【分类号】：R781.42
【正文快照】： 二肽肽酶I(DPPI,又称组织蛋白酶C)是溶酶体半胱氨酸蛋白酶家族的成员[1],在人和啮齿类动物的多种组织中都有广泛的表达[2]。DPPI具有肽链内切酶的活性,可以切除肽链末端的二肽,对于蛋白质的胞内降解过程具有必不可少的作用。近年来,越来越多的研究证实DPPI对于诸多免疫炎症相

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