咬肌力对大鼠下颌骨及髁突生长发育的影响

发布时间：2018-01-05 14:42

本文关键词：咬肌力对大鼠下颌骨及髁突生长发育的影响　出处：《南昌大学》2015年硕士论文　论文类型：学位论文

更多相关文章： 曲安奈德 髁突 下颌骨 咬肌 IGF-Ⅰ

【摘要】：目的:通过两种不同的方法处理咬肌,建立大鼠咬肌力减弱及咬肌力增强的动物模型,并观察这两种方法对大鼠髁突及下颌骨生长的影响。方法:48只21日龄SD大鼠,随机分为3组,每组16只,于室内温度22℃、相对湿度为50%~60%、气流速度≤0.2、氨浓度≤14mg/m3、噪声≤60dB条件下喂养一周后,进行试验。A组咬肌力增强组:大鼠咀嚼巧克力浸泡的木块,以增强大鼠咬肌力量;B组咬肌力减弱组:采用醋酸曲安奈德0.01ml/100g处理大鼠咬肌,以使大鼠咬肌力减弱;C组对照组,不作任何处理。在建模第3周,第6周后每组分别处死大鼠8只,分别行下颌骨形态学测量及髁突组织学检测,并进行胰岛素样生长因子-Ⅰ(Insulin like growth factor-Ⅰ,IGF-Ⅰ)表达含量的检测,比较其在不同组及同组不同时间点表达的变化。结果:本实验成功建立了大鼠咬肌力增强及咬肌力减弱的实验模型。⑴下颌骨形态学测量显示:①:下颌骨长度测量结果显示:B组(咬肌力减弱组)大鼠下颌骨长度明显小于A组(咬肌力增强组)大鼠下颌骨长度;C组(对照组)大鼠下颌骨长度明显小于A组(咬肌力增强组)大鼠下颌骨长度;C组(对照组)大鼠下颌骨长度大于B组(咬肌力减弱组)大鼠下颌骨长度,三者两两比较,差别有统计学意义(p0.05)。②:下颌升支高度测量结果显示:A组大鼠下颌支高度大于B组与C组大鼠下颌支高度;C组大鼠下颌支高度要明显大于B组大鼠下颌支高度,差别有统计学意义(p0.05)。③:下颌角角度测量结果显示:A组大鼠下颌角度大于C组大鼠下颌角度,C组大鼠下颌角度大于B组大鼠下颌角度,差别有统计学意义(p0.05)。④:下颌体高度测量结果显示:A组,B组,C组两两比较,差别无统计学意义(p0.05)。⑵髁突组织学检测结果显示:①:咬肌力增强组大鼠髁突软骨各细胞层分层明显,细胞排列密集;②:咬肌力减弱组大鼠髁突软骨各细胞层细胞排列稀疏;③:对照组髁突软骨各细胞层排列稀疏,相对于咬肌力减弱组而言,细胞排列较密集。⑶髁突软骨免疫组化结果显示,IGF-Ⅰ主要表达于成软骨细胞层和肥大细胞层。IGF-1蛋白半定量结果显示:IGF-1因子在髁突软骨细胞中的表达率,增殖层,成软骨细胞层,肥大层阳性细胞表达术后三周要强于术后六周;IGF-1因子在增殖层的表达表现为同一时刻A、B、C三组表达有差异,咬肌力增强组的表达强于对照组,对照组强于咬肌力减弱组。结论:1:在本实验环境条件下,咬肌力大时,有利于髁突及下颌骨的生长,并使下颌角角度减小。咬肌力减弱时,下颌角角度增大。2:在本实验环境条件下,髁突软骨各细胞层细胞的分化与压力的大小呈正相关。3:IGF-Ⅰ因子作为一种生长因子,在生长发育期间,应力越大,IGF-Ⅰ因子阳性表达率越高,IGF-Ⅰ因子可以作为检测髁突生长发育的指标。
[Abstract]:Objective: through two different methods of processing the masseter muscle, muscle weakness and a bite bite strength of the animal model of rats, and observe the effects of the two methods on the growth of condyle and mandible in rats. Methods: 48 21 day old SD rats were randomly divided into 3 groups, 16 rats in each group, in the room temperature of 22 degrees Celsius, relative humidity is 50%~60%, flow rate is less than 0.2, the ammonia concentration is less than or equal to 14mg/m3, a week after the noise is less than or equal to 60dB under the condition of feeding, test group.A bite strength group: rats were chewing chocolate soaked wood, in order to enhance the strength of masseter muscle weakness; group B group: bite by acetic acid 0.01ml/100g triamcinolone treated rats masseter muscle, to make rat bite muscle weakness; group C control group, without any treatment. In third weeks, sixth weeks after modeling, rats in each group were sacrificed at 8, respectively, the Mandible Condyle morphology measurement and histological examination, and insulin-like growth factor-I (Insu Lin like growth factor- I, IGF- I) expression were detected, comparing the changes in different groups and different time points in group expression. Results: we have successfully established experimental model of rat bite bite strength and muscle weakness. The mandibular morphology measurement showed: 1: the measurement results showed that the length of mandible B group (bite muscle weakness group) rat mandibular length is less than the A group (bite strength group) rat mandibular length; C group (control group) the length of mandible in rats were significantly less than group A (bite strength group) rat mandibular length; C group (control group) of rat mandibular length is greater than the B group (bite muscle weakness group) rat mandibular length, 22 compared with three, the difference was statistically significant (P0.05). The mandibular ramus height measurement results showed that A group rats mandibular ramus height greater than B group and C group rat mandibular ramus height; C rats mandibular ramus height to Ming Rats in B group were significantly larger than the height of the mandibular ramus, the difference was statistically significant (P0.05). The mandibular angle measurement results showed that: A rats mandibular angle is greater than the C rats C rats mandibular angle, mandibular angle is greater than the group B rat mandibular angle, the difference was statistically significant (P0.05). 4. The results show: mandibular height measurement: A group, B group, C group 22, the difference was not statistically significant (P0.05). The condylar histological examination of the results showed that: bite strength in condylar cartilage of rats in all cell layers increased, cells arranged densely; the bite muscle weakness of condyle cartilage of rats at each cell layer cells are sparse; the control group: the condylar cartilage layers are sparse, with respect to bite muscle weakness group, cells arranged indense. The condylar cartilage immunohistochemistry results showed that IGF- I mainly expressed in chondrogenic cell layer and the layer of.IGF-1 protein half mast cells The quantitative results showed that the expression of factor IGF-1 in condylar cartilage cells proliferation rate, layer, chondroblast layer, hypertrophic layer positive cells after three weeks than 6 weeks after surgery; IGF-1 factor showed the same time A expression in proliferative layer B, C three groups with different expression, bite strength enhanced expression of strong group than in the control group, the control group is stronger than the bite muscle weakness group. Conclusion: 1: under the experimental conditions, bite strength, is conducive to the growth of condyle and mandible, and the mandibular angle decreases. Bite muscle weakness, mandibular angle increased.2: in this experimental environment under the size of.3:IGF- was positively related to differentiation factor and pressure of condylar cartilage layers of cells as a growth factor in the growth period, the greater the stress factor, IGF- positive expression rate is high, the IGF- factor can be used to measure the condylar growth index.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R783.5

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