口腔鳞癌细胞分泌的胞外体对其生物学行为的影响

发布时间：2018-01-07 00:16

本文关键词：口腔鳞癌细胞分泌的胞外体对其生物学行为的影响　出处：《口腔医学研究》2016年08期 　论文类型：期刊论文

【摘要】：目的:研究口腔鳞状细胞癌细胞细胞株(OSC-4)分泌的胞外体对细胞生物学行为的影响。方法:培养OSC-4,从其上清培养液中分离提纯胞外体(exosome,EXO),CD9标记,将提取的胞外体作用于OSC-4,MTT检测胞外体对OSC-4增殖能力的影响,细胞划痕实验检测胞外体对OSC-4迁移能力的影响,transwill小室侵袭实验检测胞外体对OSC-4侵袭能力的影响。结果:细胞上清液提取物免疫金标结果可见金标记的CD9,即分离提纯物为胞外体;MTT检测结果显示胞外体作用后的OSC-4的增殖能力增强(P0.05),细胞划痕实验结果显示其迁移能力增强和transwill小室侵袭实验结果显示其侵袭能力也增强(P0.05)。结论:OSC-4分泌的胞外体可以促进口腔鳞状细胞癌细胞的增殖、迁移、浸润能力。
[Abstract]:Objective: to study the effect of extracellular bodies secreted by oral squamous cell carcinoma cell line (OSC-4) on cell biological behavior. Methods: OSC-4 was cultured. The exosome-exosome-exosome-exon CD9 was isolated from the supernatant of the supernatant and the extracellular bodies were used to act on OSC-4. The effects of extracellular bodies on the proliferation of OSC-4 and the effects of extracellular bodies on the migration of OSC-4 were detected by MTT and scratch assay. Transwill chamber invasion assay was used to detect the effect of extracellular bodies on the invasiveness of OSC-4. Results: the gold-labeled CD9 could be seen from the gold-labeled cells supernatant extract. That is to say, the purified substance is extracellular. The results of MTT showed that the proliferative ability of OSC-4 was enhanced by extracellular bodies (P0.05). The results of cell scratch test showed that its migration ability was enhanced and that of transwill chamber invasion test showed that it also enhanced its invasion ability (P0.05). Conclusion the extracellular body secreted by OSC-4 can promote the proliferation of oral squamous cell carcinoma cells. Migration, infiltration ability.
【作者单位】：佳木斯大学附属口腔医院颌面外科;高知医科大学口腔颌面外科;
【基金】：黑龙江省卫生厅课题(项目编号:2013-218)
【分类号】：R739.8
【正文快照】： 2.高知医科大学口腔颌面外科高知,783-8505)口腔颌面部的恶性肿瘤以鳞癌最常见,多发于40~60岁的成人,男性多于女性。鳞癌常向区域淋巴结转移,晚期可发生远处转移。口腔鳞状细胞癌能以胞外分泌的方式释放胞外体,其组成成分随细胞来源不同而有所差异[1]。胞外体的形成都需要热休

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