肿瘤相关成纤维细胞对舌鳞癌细胞功能的影响及机制研究

发布时间：2018-01-09 04:12

本文关键词：肿瘤相关成纤维细胞对舌鳞癌细胞功能的影响及机制研究　出处：《中国免疫学杂志》2015年04期 　论文类型：期刊论文

【摘要】：目的:探讨肿瘤相关成纤维细胞(CAFs)对口腔癌细胞增殖、迁移、侵袭的影响,初步分析产生影响的可能原因。方法:体外培养CAFs、舌癌细胞株SCC-9和CAL-27,并建立CAFs与舌癌细胞株共同培养体系;采用红色荧光蛋白标记法观察CAFs对舌癌细胞株增殖能力的影响;利用Transwell小室建立CAFs与舌癌细胞株SCC-9或CAL-27的交互作用模型,观察CAFs对舌癌细胞株迁移和侵袭能力的影响;采用细胞因子芯片检测CAFs单独培养、SCC-9单独培养、CAFs和SCC-9共同培养上清中可溶性细胞因子水平的差异。采用裸鼠成瘤实验观察CAFs对舌癌成瘤能力的影响。结果:培养第5天SCC-9与CAFs共同培养组中SCC-9的数量是初始SCC-9量的(4.41±0.38)倍,明显高于SCC-9单独培养组(3.21±0.35)倍;CAL-27组结果相似。24 h后SSC-9+CAFs组迁移到小室底面的SCC-9细胞与SCC-9单独培养组相比比值为2.6±0.42,CAL-27组比值为3.11±0.46。细胞侵袭研究结果与迁移研究结果类似,共同培养组穿透基质胶的OCC细胞明显增多(P0.05)。细胞因子抗体芯片分析发现,与细胞单独培养相比,共同培养组培养上清中CCL2、CCL5、IL8水平明显升高。裸鼠成瘤实验结果发现,6周后,SCC-9与CAFs混合注入组的肿瘤体积明显大于SCC-9单独注入组。结论:口腔CAFs能促进舌癌细胞株SCC-9和CAL-27的增殖、迁移和侵袭和肿瘤生长,癌细胞功能的改变可能与微环境中CCL2、CCL5、IL8等细胞因子水平升高有关。
[Abstract]:Objective: To investigate the effects of tumor associated fibroblasts (CAFs) on the proliferation of oral cancer cell migration, invasion, influence, preliminary analysis of possible causes effect. Methods: CAFs were cultured in vitro, SCC-9 and CAL-27 in tongue carcinoma cell line, and the establishment of CAFs and Tca8113 cells co culture system; the influence of red fluorescent protein CAFs was observed the tongue cancer cell proliferation ability; the interaction model of Transwell by an CAFs and oral squamous cell carcinoma cell line SCC-9 or CAL-27, the effect of CAFs on migration and invasion of carcinoma cell line; using cytokine microarray to detect CAFs cultured alone, SCC-9 cultured alone, CAFs and SCC-9 in Co culture differences in soluble cytokine levels in the supernatant the tumor formation in nude mice. The experimental observation of CAFs tumorigenicity of tongue cancer. Results: cultured for fifth days SCC-9 and CAFs in co culture group in the number of SCC-9 is the initial amount of SCC-9 (4.41 + 0.38)%, significantly higher than that of SCC-9 cultured alone group (3.21 + 0.35) times; CAL-27 group was similar to the results of.24 h SSC-9+CAFs group migrated to the bottom surface of the SCC-9 cells and SCC-9 cells were cultured alone compared ratio was 2.6 + 0.42, the ratio of group CAL-27 was 3.11 + 0.46. cell invasion and migration of the results of the study results similarly, in co culture group through Matrigel of OCC cells increased significantly (P0.05). The cytokine antibody microarray analysis showed that compared with cells cultured alone and co cultured group in culture supernatant of CCL2, CCL5, IL8 levels were significantly increased. The nude mice experiment results showed that after 6 weeks, SCC-9 and CAFs mixed injection group the tumor volume was significantly higher than that of SCC-9 single injection group. Conclusion: oral CAFs can promote SCC-9 and CAL-27 tongue cancer cell proliferation, migration and invasion of cancer cells and tumor growth, changes in CCL2, and in the micro environment of CCL5, IL8 and other cytokines The rise is related.

【作者单位】：佛山科学技术学院医学院口腔系·附属佛山市口腔医院;中南大学湘雅医学院附属肿瘤医院耳鼻喉头颈外科;
【基金】：佛山科学技术学院博士启动项目资助
【分类号】：R739.86
【正文快照】： 口腔癌(Oral carcinoma)是头颈部常见的恶性肿瘤,约占全身恶性肿瘤的3%,也是世界上排名第六的最常见的恶性肿瘤[1]。研究表明肿瘤的发生发展过程是肿瘤细胞与其相邻多种基质细胞相互作用的过程,基质细胞包括纤维母细胞、内皮细胞和多种骨髓来源的祖细胞等。其中肿瘤相关成纤

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