新疆墨玉县维吾尔族成人慢性牙周炎与维生素D受体基因多态性的相关性研究

发布时间：2018-01-11 09:25

本文关键词：新疆墨玉县维吾尔族成人慢性牙周炎与维生素D受体基因多态性的相关性研究　出处：《口腔医学研究》2015年10期 　论文类型：期刊论文

【摘要】：目的:探讨维生素D受体(vitamin D receptor,VDR)基因BsmI、TaqI位点单核苷酸多态性(Single Nucleotide Polymorphisms,SNP)与新疆墨玉县维吾尔族成人慢性牙周炎(chronic periodontitis,CP)的相关性。方法:选取2013年3~4月课题组对新疆墨玉县维吾尔族进行的流行病学调查资料及采集的颊黏膜拭子样本,共纳入185例个体,包括CP患者95例和健康对照者90例,采用聚合酶链式反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法测定BsmI、TaqI位点基因型,探讨VDR基因多态性与CP的相关性。结果:对照组和CP组在BsmI位点的基因型分布有显著性差异,等位基因频率的分布亦有显著性差异(P=0.016,P=0.003);在TaqI位点两组间的基因型和等位基因频率的分布差异均无统计学意义(P=0.582,P=0.606);在男性和女性研究对象中两组间仅BsmI位点等位基因频率的分布差异有统计学意义(P=0.025,P=0.042);单因素logistic回归分析显示bb基因型和等位基因b可能是CP的易感因子。结论:新疆墨玉县维吾尔族成人慢性牙周炎的易感性可能与VDR基因BsmI位点相关,bb基因型和等位基因b可能是维吾尔族成人CP的易感因子。
[Abstract]:Objective: to investigate the BsmI gene of vitamin D receptor (VDR). The single nucleotide polymorphism of TaqI locus was single Nucleotide Polymorphisms. SNPs and chronic periodontitis of Uygur adults in Moyu County, Xinjiang. Methods: 185 samples of buccal mucosal swabs were collected from Uygur nationality of Moyu County Xinjiang from 2013 to April. There were 95 patients with CP and 90 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism (. Polymerase chain reaction-restriction fragment length polymorphism. The genotypes of BsmI TaqI locus were determined by PCR-RFLP. To investigate the correlation between VDR gene polymorphism and CP. Results: the genotype distribution of BsmI locus was significantly different between the control group and CP group. There was also a significant difference in the distribution of allelic frequencies (P0. 016, P0. 003). There was no significant difference in the distribution of genotype and allele frequency between the two groups at TaqI locus. In male and female subjects, there were significant differences in the distribution of allelic frequencies of only BsmI locus between the two groups (P = 0.025, P = 0.042). Univariate logistic regression analysis showed that BB genotype and allele b might be the susceptible factors of CP. The susceptibility to chronic periodontitis of Uygur adults in Moyu County Xinjiang may be related to the BsmI locus of VDR gene. BB genotype and allele b may be the susceptible factors of CP in Uygur adults.
【作者单位】：新疆医科大学第一附属医院牙体牙髓科;新疆维吾尔自治区口腔医学研究所;
【基金】：中华口腔医学会西部行口腔医学临床科研基金(编号:CSA-W2014-01)
【分类号】：R781.4
【正文快照】： 慢性牙周炎(chronic periodontitis,CP)是一种由细菌刺激引起的、多因素联合参与的慢性炎症性疾病,以局部牙槽骨的吸收为主要特征,是最常见的骨病之一[1],同时也是造成我国成年人失牙的首要原因。维生素D受体(vitamin D receptor,VDR)是介导维生素D的主要活性代谢产物1,25-(OH

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