壳聚糖与氟化钠对变形链球菌生物活性影响的对比研究

发布时间：2018-01-12 03:11

本文关键词：壳聚糖与氟化钠对变形链球菌生物活性影响的对比研究　出处：《南昌大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:本实验通过在体外环境下培养变形链球菌S.MutansATCC25175,初步观察并检测壳聚糖(Chitosan,CTS)对S.Mutans的抑制作用,并与氟化钠(Sodium fluoride,NaF)的抑菌效果作对比,为壳聚糖的临床应用提供实验依据。方法:(1)应用不同浓度的CTS与NaF,观察其对S.mutans的抑制效果。实验分成三组:对照组(0.9%生理盐水)、CTS 组(0.01%,0.03%,0.05%,0.1%,0.3%,0.5%)和 NaF 组(0.01%,0.03%,0.05%,0.1%,0.3%,0.5%)。恒温厌氧环境下培养S.Mutans 48h,观察各组细菌生长状况,定性检测药物最小抑菌浓度值(minimal inhibition concentration,MIC);(2)MTT法检测48h时不同浓度条件一下的细菌吸光度值,判断细菌活力及增值;(3)使用PH检测仪检测48h时各组培养液PH的变化,计算APH。实验数据导入SPSS19.0软件进行统计分析与处理。结果:(1)菌落平板计数法显示CTS组与NaF组细菌数量均减少,NaF的MIC为 0.05%,CTS 的 MIC 为 0.5%;(2)MTT结果显示CTS组OD值小于对照组,差异有统计学意义(P0.05),而CTS组与NaF组之间无统计学差异(P＞0.05);(3)CTS与NaF两药物组实验前后△PH的变化减小,对比两种药物,差异无统计学意义(P0.05)。结论:(1)CTS与NaF均有抑制S.Mutans生长的作用且抑菌效果具有浓度依赖性。(2)在抑菌效果方面,CTS与NaF无差别,CTS能达到临床抑菌要求。
[Abstract]:Objective: to observe and detect chitosan by S.MutansATCC25175 in vitro. The inhibitory effect of CTS on S. Mutans was compared with that of sodium fluoride (NAF). Methods: different concentrations of CTS and NaF were used. To observe the inhibitory effect of S.mutans, the experiment was divided into three groups: control group (0.9% normal saline) and CTS group (0.01%). 0. 3: 0. 5% and 0. 01% in NaF group, 0. 05%, 0. 5% and 0. 3%. S.Mutans was cultured under constant temperature and anaerobic environment for 48 hours, and the growth of bacteria was observed in each group. The minimal inhibition concentration was detected qualitatively. MTT assay was used to detect the absorbance of bacteria at different concentration for 48 h to judge the activity and increment of bacteria. (3) using PH detector to detect the changes of PH in culture medium of each group at 48 h. The experimental data were imported into SPSS19.0 software for statistical analysis and processing. Results the colony plate counting method showed that the number of bacteria in both CTS group and NaF group was decreased. The MIC of NaF was 0. 05 and the MIC of CTS was 0. 5. The OD value of CTS group was lower than that of control group (P < 0.05), but there was no significant difference between CTS group and NaF group (P > 0.05). The changes of PH in the CTS and NaF groups were decreased before and after the experiment, and the two drugs were compared. Conclusion both CTS and NaF can inhibit the growth of S.Mutans and the bacteriostatic effect is concentration-dependent. There is no difference between CTS and NaF.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783.5

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