4NQO诱导大鼠舌黏膜癌变过程中E-cad及PCNA的表达研究

发布时间：2018-01-28 18:28

本文关键词： 舌肿瘤癌鳞状细胞动物模型 E-cad PCNA 免疫组织化学　出处：《重庆医学》2017年11期 　论文类型：期刊论文

【摘要】：目的研究上皮钙依赖黏附蛋白(E-cad)和增殖细胞核抗原(PCNA)在大鼠口腔黏膜癌变过程中的表达变化,探讨其相关性。方法采用4-硝基喹啉-1-氧化物(4NQO)饮水法诱导大鼠舌癌变模型,免疫组织化学SP法检测82只大鼠舌癌变过程不同病理阶段组织中E-cad、PCNA蛋白的表达,分析E-cad与PCNA相关性。结果在正常黏膜、上皮单纯增生、轻度上皮异常增生、中重度上皮异常增生和舌鳞状细胞癌组织中,E-cad的阳性率分别是100%、95.24%、92.86%、80.00%、68.75%,差异均有统计学意义(P0.05);PCNA的阳性率分别是9.52%、14.29%、35.71%、50%、56.25%,差异有统计学意义(χ2=16.676,P0.05)。二者的蛋白表达呈负相关(r=-0.614,P0.01)。结论 E-cad和PCNA有望成为舌黏膜癌变的生物标志物之一。
[Abstract]:Objective to study the expression of E-cadA and PCNA in the carcinogenesis of oral mucosa in rats. Methods the rat model of tongue cancer was induced by drinking water with 4-nitroquinoline-1-oxide-4NQO. Immunohistochemical SP method was used to detect the expression of E-cadPCNA protein in 82 rats with different pathological stages of tongue carcinogenesis. The correlation between E-cad and PCNA was analyzed. Results the expression of E-cadPCNA was found in normal mucosa. The positive rates of E-cad in epithelial simple hyperplasia, mild epithelial dysplasia, moderate and severe epithelial dysplasia and tongue squamous cell carcinoma were 100%, 95.24% and 92.86%, respectively. The difference was statistically significant (P 0.05). The positive rates of PCNA were 9.52 / 14.29 and 35.71and 56.25, respectively. The difference was statistically significant (蠂 2 / 16.676). Conclusion E-cad and PCNA may be one of the biomarkers of tongue carcinogenesis.
【作者单位】：西南医科大学附属口腔医院口腔黏膜科/西南医科大学口颌面修复重建和再生实验室;
【基金】：四川省科技厅课题(2014LY-62)
【分类号】：R739.86
【正文快照】： 口腔鳞状细胞癌尤其是舌鳞状细胞癌的侵袭性强,恶性度高,严重威胁患者的生命健康[1]。上皮钙依赖黏附蛋白(E-cadherin,E-cad)是存在于上皮组织中的细胞黏附分子,是肿瘤转移的抑制因素[2]。增殖细胞核抗原(proliferating cell nucle-ar antigen,PCNA)是一种存在于细胞核内的蛋

【相似文献】