FRMD4A在舌鳞状细胞癌中的表达及意义

发布时间：2018-01-29 04:49

本文关键词： 舌鳞癌 FRMDA qRT-PCR 免疫组织化学靶向治疗　出处：《广东医学》2014年01期 　论文类型：期刊论文

【摘要】：目的观察FRMD4A在口腔舌鳞状细胞癌(简称鳞癌)中的表达情况,探讨其在舌鳞癌发生发展中的作用。方法实时定量qRT-PCR检测10例舌鳞癌组织和相应10例癌旁正常舌组织中FRMD4A mRNA的表达;免疫组化检测FRMD4A蛋白在78例舌鳞癌、15例舌白斑和15例癌旁正常组织中的表达情况;t检验分析FRMD4A mRNA表达差异,醊2检验分析FRMD4A蛋白表达情况与临床病理特征之间的关系。结果 FRMD4A mRNA的表达在舌鳞癌组织中显著高于癌旁正常舌组织;FRMD4A蛋白的表达在舌鳞癌组织中明显高于舌白斑组织和癌旁正常组织;阳性表达主要在基底层,随肿瘤恶性程度增加,有向棘层扩散的趋势;FRMD4A蛋白高表达多出现于发生淋巴结转移和TNM分期晚的患者,差异有统计学意义(P0.05)。FRMD4A蛋白在舌鳞癌组织中的高表达,但与肿瘤分化程度、患者性别和年龄无关。结论 FRMD4A高表达与舌鳞癌的发展及转移有关,可作为临床预后危险因素和潜在治疗靶点。
[Abstract]:Objective to investigate the expression of FRMD4A in oral squamous cell carcinoma (SCC). Methods Real-time quantitative qRT-PCR was used to detect the expression of FRMD4A mRNA in 10 cases of tongue squamous cell carcinoma and 10 cases of adjacent normal tongue tissues. The expression of FRMD4A protein in 15 cases of tongue leukoplakia and 15 cases of adjacent normal tissues were detected by immunohistochemistry. T test was used to analyze the difference of FRMD4A mRNA expression. Results the expression of FRMD4A mRNA in tongue squamous cell carcinoma was significantly higher than that in adjacent normal tongue tissue. The expression of FRMD4A protein in tongue squamous cell carcinoma was significantly higher than that in leukoplakia and adjacent normal tissues. The positive expression was mainly located in the basal layer, with the malignant degree of the tumor increasing, there was a tendency to spread to the spinous layer. The high expression of FRMD4A protein was found in the patients with lymph node metastasis and late TNM stage, and the difference was statistically significant (P 0.05) .FRMD4A protein was highly expressed in tongue squamous cell carcinoma (TSCC). Conclusion the high expression of FRMD4A is associated with the development and metastasis of tongue squamous cell carcinoma, and may be a risk factor for clinical prognosis and a potential therapeutic target.
【作者单位】：南方医科大学附属口腔医院;广东省口腔医院口腔颌面外科;
【基金】：广东省科技计划项目(编号:2010B031500032)
【分类号】：R739.8
【正文快照】： 口腔舌鳞状细胞癌(oral tongue squamous cell car-cinoma,简称鳞癌)是口腔颌面部常见的恶性肿瘤,占头颈部恶性肿瘤的5%~15.5%。舌鳞癌的发病机制仍未研究透彻,涉及多个基因突变及表达异常。FRMD4A(FERM domain-containing protein 4A)蛋白在头颈癌中起着关键作用,FRMD4A蛋白

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