苯妥英钠对大鼠骨髓间充质干细胞与血管内皮细胞VEGF和SCF分泌的影响

发布时间：2018-01-30 23:10

本文关键词： 大鼠骨髓间充质干细胞血管内皮细胞间接共培养血管新生苯妥英钠　出处：《西安交通大学学报(医学版)》2016年02期 　论文类型：期刊论文

【摘要】：目的研究苯妥英钠(PHT)对大鼠骨髓间充质干细胞(rBMSCs)和血管内皮细胞(rVECs)间接共培养体系中血管内皮细胞生长因子(VEGF)和干细胞因子(SCF)分泌的影响。方法分别建立rBMSCs、rVECs单独培养组及共培养组,各组添加不同质量浓度的PHT(0、20、40μg/mL)。在培养第2、4、6天时,用双抗体夹心ABC-ELISA法检测培养上清中VEGF和SCF的含量。结果 1VEGF:培养第二天时,在相同PHT作用下,共培养组VEGF含量高于rBMSCs单独培养组(P0.05)和rVECs单独培养组(P0.001);共培养组随着培养时间的延长和PHT浓度增大VEGF含量增加(P0.001,P0.05)。2SCF:共培养组SCF含量高于相同PHT浓度下的单独培养组;在共培养组中,当PHT为20μg/mL时,随着培养时间延长SCF含量增加;当PHT为40μg/mL时,随着培养时间延长SCF含量减少,但差异无统计学意义(P0.05)。结论 PHT可促进rBMSCs与rVECs共培养体系中VEGF的分泌,可能降低SCF的分泌。
[Abstract]:Objective to study the phenytoin (PHT) on rat bone marrow mesenchymal stem cells (rBMSCs) and vascular endothelial cells (rVECs) indirectly co cultured vascular endothelial growth factor system (VEGF) and stem cell factor (SCF) secretion. RBMSCs method were established, rVECs were cultured alone and co cultured group, each group the addition of different concentrations of PHT (0,20,40 g/mL). In the training of the first 2,4,6 days, were measured by double antibody sandwich ABC-ELISA training content of VEGF and SCF in supernatant. Results 1VEGF: cultured for second days, in the same PHT, co culture content of VEGF was higher than that of rBMSCs (P0.05) were cultured alone and rVECs alone training group (P0.001); group with prolonged incubation time and the concentration of PHT increased the content of VEGF increased in co culture (P0.001, P0.05).2SCF: co culture content of SCF group was higher than that of single culture group under the same PHT concentration; in the co culture group, when the PHT is 20 g/mL, with the The SCF content increased with the prolonging of culture time. When PHT was 40 g/mL, SCF content decreased with the prolongation of culture time, but the difference was not statistically significant (P0.05). Conclusion PHT can promote the secretion of VEGF in rBMSCs and rVECs co culture system, and may reduce the secretion of SCF.

【作者单位】：西安交通大学第二附属医院口腔科;广州市第一人民医院口腔科;西安高新医院口腔科;西安交通大学口腔医院牙周黏膜科;
【基金】：陕西省科技厅社发攻关资助项目(No.2012SF2-22)~~
【分类号】：R781.4
【正文快照】： 目前,牙周损伤区再生修复的研究大多集中于干细胞向牙周各类成体细胞的增殖分化方面,对于血管新生方面的研究相对较少,而血管新生对促进损伤区的再生修复非常重要。研究提示除了牙周局部的前体细胞外,来自骨髓和血液的间充质细胞也可能参与损伤区的血管新生过程[1]。苯妥英钠(

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