人颌骨间充质干细胞的外泌体在巨噬细胞调控中的作用

发布时间：2018-02-02 05:08

本文关键词： 颌骨间充质干细胞(OMMSCs) 外泌体(Exo) miRNA 巨噬细胞　出处：《实用口腔医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:探讨颌骨骨髓间充质干细胞分泌的外泌体(OMMSCs-Exo)的特性及其在调控巨噬细胞功能中可能发挥的作用。方法:体外培养OMMSCs,检测其克隆形成及多向分化能力。提取上清液中外泌体,透射电镜观察其形态,蛋白印迹法检测其表面特异标志CD63、CD81。将外泌体与巨噬细胞作用24 h后用共聚焦显微镜观察两者的作用方式,实时定量PCR检测外泌体内免疫相关miR-223和miR-let-7c表达及与共培养后巨噬细胞中miRNA表达。结果:人OMMSCs符合间充质干细胞特性,OMMSCs-Exo分泌的外泌体近似圆形,直径在60~160 nm之间,表达表面标志CD63、CD81:内含有与巨噬细胞调控相关的miRNA如miR-223和miR-let-7c等,能被巨噬细胞吞噬,共培养后巨噬细胞中miR-223含量增加。结论:人OMMSCs-Exo及其携带的miRNA可能参与了巨噬细胞功能的调控。
[Abstract]:Objective: to investigate the characteristics of OMMSCs-Exo) secreted by bone marrow mesenchymal stem cells (BMSCs) and its role in regulating the function of macrophages. Methods: OMMSCs was cultured in vitro. The ability of clone formation and multidirectional differentiation was detected. The supernatant secretions were extracted. The morphology of the supernatant was observed by transmission electron microscope. The surface specific marker CD63 was detected by Western blot. CD81.After the exocrine and macrophage were treated for 24 h, the mode of action was observed by confocal microscope. Real-time quantitative PCR was used to detect the expression of immune-related miR-223 and miR-let-7c in vitro and the expression of miRNA in macrophages after co-culture. Human OMMSCs accorded with the characteristics of mesenchymal stem cells. The exocrine body secreted by OMMSCs-Exo was approximately circular, with a diameter of 60 ~ 160nm, and expressed the surface marker CD63. CD81: it contains miRNA related to macrophage regulation, such as miR-223 and miR-let-7c, which can be swallowed by macrophages. Conclusion: human OMMSCs-Exo and its miRNA may be involved in the regulation of macrophage function.
【作者单位】：广州医科大学附属第一医院口腔科;
【基金】：广州市科信局科技惠民专项资助(编号:2014Y2-00058) 广州市属高校科研计划科技类一般项目(编号:2012C096)
【分类号】：R782
【正文快照】： 颌骨来源的间充质干细胞(orofacial bone-marrow-derived MSCs,OMMSCs)可以从颌面部整形外科手术废弃的颌骨碎片获得,且OMMSCs增殖及成骨能力均强于长骨来源的间充质干细胞(BMMSCs)[1]。间充质干细胞具有多向分化能力并且具有免疫调节的能力,可调控巨噬细胞的功能[2-3],骨髓间

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