新型钛种植体涂层构建及体外生物安全性评价

发布时间：2018-02-03 05:39

本文关键词： 钛种植体载药微球涂层生物安全性　出处：《实用口腔医学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:构建一种新型钛种植体涂层,检测其表面形貌及体外生物安全性。方法:采用明胶作为交联剂,以本课题组前期制备的包裹SDF-1和rhBMP-2的双层载药纳米微球为基础,通过振荡渗涂交联法将微球交联至微弧氧化涂层的微孔中,在钛种植体表面构建出新型功能性涂层,观察其表面形貌。通过细胞毒性试验、口腔黏膜刺激试验及溶血试验对新型钛种植体涂层体外生物安全性进行初步检测。结果:扫描电镜结果显示新型功能性涂层形貌良好。细胞毒性试验结果显示该涂层100%浸提液细胞毒性为1级,溶血率为4.6%,口腔黏膜刺激试验结果显示该涂层对黏膜无刺激作用。结论:通过振荡渗涂交联法构建的新型钛种植体表面涂层具有良好的表面形貌及生物安全性。
[Abstract]:Objective: to construct a new titanium implant coating and to detect its surface morphology and biological safety in vitro. Methods: gelatin was used as crosslinking agent. Based on the double-layer drug-loaded nano-spheres coated with SDF-1 and rhBMP-2 prepared by our team, the microspheres were cross-linked into the micropores of the micro-arc oxidation coatings by oscillatory permeation and cross-linking. A new functional coating was constructed on the surface of titanium implant and its surface morphology was observed. Oral mucosal irritation test and hemolysis test were used to detect the biological safety of the new titanium implant coating in vitro. The results of scanning electron microscope showed that the morphology of the new functional coating was good, and the cytotoxicity test of the coating 100% showed that the cytotoxicity of the coating was grade 1. The hemolysis rate was 4.6%. The results of oral mucosal irritation test showed that the coating had no irritation effect on the mucous membrane. Conclusion: the new titanium implant coating prepared by oscillatory osmosis and cross-linking method has good surface morphology and biological safety.
【作者单位】：军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔医学重点实验室第四军医大学口腔医院修复科;战略支援部队航天系统部航天门诊部;
【基金】：国家自然科学基金(编号:57311006)
【分类号】：R783.1
【正文快照】： 种植体相关感染仍是导致种植失败的重要原因之一[1]。感染导致的骨吸收,种植体松动甚至脱落都是临床的难题。如何寻求有效的方法抑制骨缺损区域的骨吸收,促进骨再生是当下研究的热点[2]。基于缓释微球技术的种植体表面涂层的构建为其治疗注人了新理念,Wu等[3]在微弧氧化的纯

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