生物钟基因PER2在人口腔鳞癌细胞中具有重要的抑癌作用

发布时间：2018-02-05 19:41

本文关键词： 癌口腔生物钟 PER 肿瘤基因　出处：《基因组学与应用生物学》2017年11期 　论文类型：期刊论文

【摘要】：探讨生物钟基因PER2对人口腔鳞癌SCC9细胞增殖、凋亡、迁移和侵袭的影响和机理。利用RNA干扰技术沉默SCC9细胞中PER2基因,应用实时荧光定量PCR检测Ki-67、MDM2、P53、Bcl-2、Bax、C-myc、MMP-2、Timp-2和VEGFm RNA的表达改变;流式细胞仪检测沉默后细胞的增殖和凋亡水平,平板克隆形成实验检测细胞的克隆形成率,Transwell小室检测细胞迁移和侵袭能力的改变。沉默PER2基因后,SCC9细胞凋亡指数显著降低(p0.05),细胞增殖指数、细胞迁移和侵袭能力显著升高(均p0.05)。PER2沉默后Ki-67、MDM2、Bcl-2、C-myc、MMP-2和VEGF m RNA的表达水平显著升高(均p0.05),p53、Bax和Timp-2 m RNA的表达水平显著降低(均p0.05)。研究表明,生物钟基因PER2通过调控Ki-67、MDM2、P53、Bcl-2、Bax、C-myc、MMP-2、Timp-2和VEGF影响癌细胞的增殖、凋亡、迁移和侵袭。因此,对PER2的深入研究有可能为癌症的治疗提供新的有效分子靶点。
[Abstract]:To investigate the effect and mechanism of clock gene PER2 on the proliferation, apoptosis, migration and invasion of human oral squamous cell carcinoma (SCC9) cells. RNA interference technique was used to silence the PER2 gene in SCC9 cells. A real-time fluorescent quantitative PCR was used to detect the expression of Ki-67MDM2P53Bcl-2BaxAX C-mycfen MMP-2. The expression of Timp-2 and VEGFm RNA changed. Flow cytometry was used to detect the level of cell proliferation and apoptosis after silencing. Transwell chamber was used to detect the change of cell migration and invasion ability. After silencing PER2 gene, the apoptosis index of SCC9 cells decreased significantly, and the proliferation index of SCC9 cells decreased significantly. The ability of cell migration and invasion was significantly increased (all p0.05N. PER2 was silenced) and Ki-67MDM2m2Bcl-2c-myc was observed. The expression levels of MMP-2 and VEGF m RNA were significantly increased (p0.05). The expression levels of Bax and Timp-2 m RNA were significantly decreased (p0.05). The results showed that the biological clock gene PER2 regulated Ki-67M2MDM2P53. Bcl-2nBax-C-mycfon MMP-2Timp-2 and VEGF affect the proliferation, apoptosis, migration and invasion of cancer cells. The further study of PER2 may provide a new effective molecular target for cancer treatment.
【作者单位】：重庆医科大学附属第一医院口腔颌面外科;
【分类号】：R739.8

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