磨牙缺失对幼年大鼠海马神经元调亡及Caspase-3蛋白表达的影响

发布时间：2018-02-26 02:13

本文关键词： 牙齿缺失学习记忆海马组织细胞凋亡　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察磨牙缺失后对幼年大鼠海马CA1区神经元细胞凋亡及海马神经元中Caspase-3的表达的影响,以探讨磨牙缺失幼年大鼠海马神经细胞凋亡可能的机制。方法:雄性SD大鼠,4-5周龄60只,按完全随机设计平均分成对照组、实验组(磨牙缺失组)(n=30),再依据造模后断头取脑时间将30只大鼠随机分成4周组、8周组、20周组,采用HE染色及免疫组织化学法检测Caspase-3蛋白的表达情况及末端标记法(TUNEL)法观察大鼠脑内神经细胞凋亡的变化。结果:1.对照组海马CA1区细胞形态结构正常,无明显病理性改变。实验组细胞出现肿胀、空泡,结构排列紊乱,神经元细胞明显减少。2.在4周、8周、20周,对照组Caspase-3无统计学差异(P=0.301),实验组海马CA1区Caspase-3阳性细胞数增加,差异有统计学意义(P=0.000),任意两组相比Caspase-3表达差异均有统计学意义,P0.001;3.实验组TUNEL阳性细胞数有统计学差异(P=0.000),对照组TUNEL阳性细胞数无统计学差异(P=0.276)任意两组相比TUNEL阳性细胞数表达差异均有统计学意义(P0.001)。结论:长时期咀嚼刺激减少会导致大鼠海马区细胞凋亡增多,同时可上调Caspase-3蛋白的表达,提示长时期磨牙缺失诱导加快致发育期的大鼠海马组织细胞发生凋亡。
[Abstract]:Aim: to observe the effect of molar tooth loss on apoptosis of hippocampal CA1 neurons and expression of Caspase-3 in hippocampal neurons of young rats. To explore the possible mechanism of neuronal apoptosis in hippocampal neurons of young rats with molar absence methods: 60 male SD rats aged 4-5 weeks were divided into two groups according to the complete randomized design. In the experimental group, 30 rats were randomly divided into 4 weeks group, 8 weeks group and 20 weeks group. The expression of Caspase-3 protein and the changes of neuronal apoptosis in rat brain were detected by HE staining and immunohistochemical method. Results: 1. The morphology and structure of hippocampal CA1 cells in the control group were normal. There was no obvious pathological change. In the experimental group, there were swelling, vacuole, disorder of structure and decrease of neuronal cells. After 4 weeks, 8 weeks and 20 weeks, there was no significant difference in Caspase-3 in the control group (P < 0. 301). The number of Caspase-3 positive cells in the hippocampal CA1 area of the experimental group was increased. There was significant difference in the expression of Caspase-3 between any two groups. There was a statistical difference in the number of TUNEL positive cells in the experimental group and in the control group. There was no statistical difference in the number of TUNEL positive cells in the control group (P < 0. 276). The number of TUNEL positive cells in the experimental group was smaller than that in the TUNEL group (P < 0. 276). Conclusion: the decrease of masticatory stimulation for a long period of time will lead to the increase of apoptosis in the hippocampus of rats. At the same time, the expression of Caspase-3 protein could be upregulated, suggesting that long term molar deletion could accelerate the apoptosis of hippocampal tissue cells in developing rats.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781

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