VEGF在交感神经调节骨愈合过程中的可能作用

发布时间：2018-03-02 15:16

  本文选题：血管再生　切入点：交感神经　出处：《福建医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的:骨修复改建活动复杂而有序,与血管生成密不可分,它们相互影响和紧密联系。以往认为骨代谢的调节涉及骨局部的自分泌、旁分泌以及内分泌等因素,然而越来越多的研究指出交感神经系统在骨形成和骨吸收过程中也发挥关键的作用。本研究通过检测失交感神经支配下拔牙窝骨愈合过程中VEGF及CD31的表达特点,进一步探讨血管再生在交感神经调节骨愈合过程中的可能作用。材料和方法:利用改良颈上神经切除术方法切除SD大鼠一侧颈上神经节,建立失交感神经支配的动物模型。拔除SD大鼠失交感神经侧(SCGX侧)与交感神经支配侧(CG侧)上颌第一磨牙,建立拔牙窝骨愈合动物模型。分别于拔牙术后1、3、7、14和21天5个时间点处死动物。通过免疫组化技术检测CD31的表达,测定血管内皮细胞数目。并检测拔牙窝底1/3、中1/3、顶1/3在拔牙后不同时间点VEGF的表达水平,测定其平均光密度值,进一步探讨交感神经信号调节新骨形成和骨改建过程VEGF时空表达特点对血管再生的可能作用。结果:1、SD大鼠颈上神经节切除侧表现出明显的上睑下垂的交感神经功能抑制的症状,免疫组化染色证实切除组织为颈上神经节组织。通过拔除SCGX侧及CG侧上颌第一磨牙,成功建立拔牙窝骨愈合模型。2、CD31免疫组化检测结果表明:拔牙后第1天,SCGX组拔牙窝内CD31阳性染色的血管内皮细胞数量较CG组略多,但差异无统计学意义(P0.05);SCGX组在术后第3天、7天、14天时阳性表达的血管内皮细胞数量较CG组增加显著,两组差异有统计学意义(P0.05),在第21天时两组血管内皮细胞数量均下降,且两组数目接近,差异无统计学意义(P0.05)。3、VEGF表达的平均光密度值检测结果表明:1)拔牙窝底1/3VEGF的表达:术后第1天,SCGx组的VEGF表达较对照组略高,但差异无统计学意义(P0.05);随后VEGF表达增强,且第3天、7天、14天,两组差异具有统计学意义(P0.05),而在第21天,两组差异无统计学意义(P0.05)。2)拔牙窝中1/3VEGF的表达,SCGX组与CG组在术后第1天,表达较弱,两组之间差异无统计学意义(P0.05),在拔牙后第3天、7天、14天、21天SCGx组的VEGF表达高于CG组,差异具有统计学意义(P0.05)。3)拔牙窝顶1/3VEGF的表达,SCGX组与CG组在术后第1天,第3天,两组之间差异无统计学意义(P0.05),拔牙后第7天、14天、21天SCGx组的VEGF表达高于CG组,差异具有统计学意义(P0.05)。4)在拔牙窝VEGF整体表达来看,SCGX组与CG组在术后第1天,两组之间差异无统计学意义(P0.05),在拔牙后第3天、7天、14天、21天两组差异具有统计学意义(P0.05),整个拔牙窝的VEGF表达情况与拔牙窝中1/3相同。5)CG组的拔牙窝底部1/3与顶部1/3VEGF平均光密度比较,术后第1天、14天两组间差异没有统计学意义(P0.05),在术后第3天、7天、21天具有统计学意义(P0.05)。6)SCGX组的SD大鼠拔牙窝底部1/3与顶部1/3VEGF平均光密度比较,在术后第1天、3天、7天、21天,两位点间差异有统计学意义(P0.05),而14天,两位点差异无统计意义(P0.05)。结论:失交感神经支配调节拔牙窝愈合新骨形成的过程,可能是通过促进拔牙窝VEGF的表达,促进拔牙窝新骨形成区毛细血管新生,增加血管数量来改善拔牙窝的供血以改善微循环,从而促进新骨形成。
[Abstract]:Objective: bone repair and reconstruction of complex and orderly, and angiogenesis are closely related, they affect each other and close contact. In the past that the regulation of bone metabolism involves autocrine bone, paracrine and endocrine factors, but more and more research is also pointed out that play a key role in the absorption process of the sympathetic nervous system in bone formation and bone. Expression of VEGF and CD31 in this study through the detection of sympathetic denervation by extraction socket healing process, to further explore the possible role in regulating bone vascular regeneration of sympathetic nerve in the healing process. Materials and methods: the cervical nerve resection by resection of SD rats to establish animal model of superior cervical ganglion, loss of sympathetic the innervation of the sympathetic nerve loss. Removal of SD rat side (SCGX side) and the sympathetic innervation side (CG side) of maxillary first molar, the establishment of the animal model of bone healing extraction sockets . respectively after extraction of 1,3,7,14 and 21 days 5 time points were animal. Using immunohistochemistry to detect the expression of CD31, vascular endothelial cell number and detection. The sockets at the bottom of the 1/3, 1/3, top 1/3 in the extraction at different time points after the expression of VEGF, determine the average optical density value, further study of sympathetic signal regulation of new bone formation and remodeling of the spatial and temporal expression of VEGF characteristics of the possible role of vascular regeneration. Results: 1 SD rat superior cervical ganglion resection side showed obvious function of sympathetic nerve ptosis inhibitory symptoms, immunohistochemical staining for tissue resection of superior cervical ganglion by tissue. Remove the SCGX side and the CG side of the maxillary first molar, successfully established the extraction socket healing model.2, CD31 immunohistochemistry results showed that: first days after extraction, SCGX extraction fossa CD31 positive staining the number of endothelial cells was lower than that of CG 缁勭暐澶，

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