IL-21R在舌鳞状细胞癌中的表达及意义

发布时间：2018-03-03 11:45

本文选题：舌癌　切入点：炎症免疫　出处：《免疫学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的炎症通过其对肿瘤微环境的影响在癌症的发展过程中起着重要作用。然而炎症与肿瘤之间的关系仍然需要进一步阐明。本研究主要观察IL-21R在舌鳞状细胞癌的表达和分布情况。方法使用逆转录酶聚合酶链反应(RT-PCR)、Western blot免疫印迹方法检测来自54个舌鳞状细胞癌患者手术标本IL-21R的基因和蛋白表达,并且观察IL-21R表达与临床病理特征的关系。同时,进一步通过免疫组织化学和免疫荧光技术观察IL-21R表达与分布规律。结果 RT-PCR和Western印迹分析的结果表明,与癌旁组织相比,舌鳞状细胞癌标本中IL-21R基因和蛋白表达显著降低(P0.05)。IL-21R低表达与性别、年龄、分化程度、肿瘤大小均无密切关系。这种表达特点主要与临床分期呈正相关。免疫组织化学结果显示,IL-21R主要表达于舌癌旁组织鳞状细胞和上皮细胞,而鳞状细胞癌组织中表达较弱或散在的表达。IL-21R与IL-10的双染荧光结果显示,IL-21R阳性的细胞中可见IL-10的阳性染色。结论这些数据表明IL-21R在舌癌肿瘤发生与侵袭性过程中可能起着重要作用。
[Abstract]:The purpose of inflammation through its impact on the tumor microenvironment in cancer development plays an important role. However, the relationship between inflammation and cancer remains to be elucidated. This study is designed to investigate the expression and distribution of IL-21R in tongue squamous cell carcinoma. Methods using reverse transcriptase polymerase chain reaction (RT-PCR), gene and protein expression detection of Western blot by Western blot method from 54 surgical specimens of patients with tongue squamous cell carcinoma cell line IL-21R, and observe the relationship between IL-21R expression and clinicopathological features. At the same time, further through the observation of IL-21R chemical and immunofluorescence techniques in immune tissues and distribution. The results of RT-PCR and Western blot analysis showed that compared with the adjacent tissues. Expression of IL-21R in specimens of squamous cell carcinoma of the tongue gene and protein significantly decreased (P0.05) low expression of.IL-21R and gender, age, differentiation degree, tumor large Small had no close relationship. The expression characteristics and clinical stage were positively correlated. Immunohistochemical results showed that IL-21R was mainly expressed in tissues of squamous cell carcinoma of tongue and epithelial cells, squamous cell carcinoma and expression of.IL-21R and IL-10 is weak or scattered in the fluorescent double staining showed that positive staining of IL-10 showed IL-21R positive the cells. Conclusion these data suggest that IL-21R may play an important role in tumorigenesis and invasive squamous cell carcinoma of the tongue in the process.

【作者单位】：重庆医科大学附属第一医院口腔科;
【分类号】：R739.86

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