小鼠牙胚在肾包膜及口腔黏膜环境下成牙能力的比较研究

发布时间：2018-03-04 18:25

本文选题：组织工程　切入点：牙胚　出处：《中国病理生理杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的:通过对比分析肾包膜和口腔黏膜环境对小鼠牙胚成牙的适宜度,期望为牙齿再生技术探寻较为理想的培养环境。方法:构建小鼠牙胚肾包膜下及口腔黏膜下移植模型,将14.5 d胚龄(ED14.5)的胚鼠下颌第1磨牙牙胚分别移入肾包膜及口腔黏膜下,于植入后3、4周取材,进行大体标本观察、组织学切片分析、硬度、弹性模量测定及拉曼光谱分析等,以观察牙胚的成牙情况。结果:(1)牙胚在肾包膜下及口腔黏膜下均可发育成牙齿状结构,但口腔黏膜组在各时点牙冠体积均小于肾包膜组,且牙齿尖窝发育状态不如肾包膜组明显。(2)组织切片染色显示:在口腔黏膜下形成的牙釉质层和牙本质层比肾包膜组薄,成釉细胞及成牙本质细胞分化不明显。(3)在釉质的硬度比较上,仅口腔黏膜4周组的釉质硬度比正常鼠牙低(P0.05);在釉质的弹性模量比较上,肾包膜下3周后的移植物釉质弹性模量比成年鼠牙略低,但差别无统计学意义(P0.05),肾包膜下4周和口腔黏膜下4周后的移植物釉质弹性模量均低于正常鼠牙和肾包膜3周的釉质弹性模量(P0.01);各组间牙本质的硬度及弹性模量无显著差异(P0.05)。(4)拉曼光谱分析结果显示:2组的拉曼光谱趋势基本一致,在961 cm-1处有最大峰值,但是在2 947 cm-1处,口腔黏膜3周组有明显的峰值,远高于其它各组。结论:(1)对于ED14.5的牙胚来说,相对口腔黏膜环境而言,肾包膜下环境培育3~4周更能发育成相对完整的牙齿。(2)口腔黏膜环境所形成牙齿的诸多性状与肾包膜环境下发育的牙齿相比虽有差异,但其对牙胚的生长发育还是具有一定的影响。
[Abstract]:Objective: to compare and analyze the suitability of renal capsule and oral mucosal environment to tooth formation of mouse tooth germ, and to explore an ideal culture environment for tooth regeneration. Methods: a model of subcapsular and oral submucosal transplantation of mouse tooth germ was established. The mandibular first molar tooth germs of 14.5 d (ED14.5) were transplanted into the renal capsule and oral mucosa respectively. The specimens were collected at 3 and 4 weeks after implantation, and the specimens were observed, histologically and sectioned, hardness, elastic modulus and Raman spectroscopy were measured. Results the dental germs could develop into dentate structures under the renal capsule and under the oral mucosa, but the crown volume in the oral mucosa group was smaller than that in the renal capsule group at each time point. The developmental state of the apical fossa of the teeth was not as obvious as that of the renal capsule group. The staining showed that the enamel layer and dentin layer formed under the oral mucosa were thinner than those in the renal capsule group. Ameloblast and odontoblast differentiation were not obvious. The enamel hardness was lower in the oral mucous membrane group than that in the normal rat tooth, and the elastic modulus of the enamel was lower than that in the oral mucous membrane group after 4 weeks. The elastic modulus of the grafts after 3 weeks under the renal capsule was slightly lower than that of the adult rat teeth. But there was no significant difference (P 0.05). The elastic modulus of graft enamel in 4 weeks under renal capsule and 4 weeks in oral mucosa was lower than that in normal rat teeth and renal capsule at 3 weeks, and the hardness and elastic modulus of dentin in each group was lower than that in normal mouse teeth and renal capsule at 3 weeks. The results of Raman spectroscopy analysis showed that the Raman spectra of the two groups were basically the same. There was a peak value at 961 cm-1, but at 2 947 cm-1, the peak value of oral mucosa in the 3-week group was much higher than that in the other groups. Conclusion: 1) for the tooth germ of ED14.5, it is relative to the oral mucosal environment. The teeth formed in the oral mucosal environment were different from those developed in the renal capsule environment, although they were different from those developed in the renal capsule environment, which were more likely to develop into relatively complete teeth after 3 weeks of incubation under the renal capsular environment, although many of the characteristics of the teeth formed in the oral mucosal environment were different. But it still has certain influence on the growth and development of tooth germ.
【作者单位】：暨南大学医学院口腔医学系;吉林大学药学院再生医学系;中国科学院广州生物医药与健康研究院再生生物学重点实验室;
【基金】：中国科学院广州生物医药与健康研究院再生生物学重点实验室开放课题资助项目(No.KLRB201217)
【分类号】：R782.1

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