DNMT1、PDCD4在舌鳞状细胞癌中的表达及临床意义

发布时间：2018-03-05 06:52

本文选题：DNA甲基转移酶　切入点：程序性细胞死亡因子　出处：《重庆医学》2014年31期 　论文类型：期刊论文

【摘要】：目的探讨DNA甲基转移酶1(DNMT1)、程序性细胞死亡因子4(PDCD4)在舌鳞状细胞癌(TSCC)组织中的表达及临床意义。方法采用免疫组织化学Elivision二步法检测40例TSCC组织及其相对应的癌旁非肿瘤组织中DNMT1、PDCD4蛋白表达水平,并分析两者蛋白表达与临床病理参数的关系。结果在TSCC组织中DNMT1蛋白呈高表达,而PDCD4蛋白呈低表达甚至不表达;两者在癌旁非肿瘤组织中的表达则与之相反。两者的表达呈明显的负相关(r=-0.452,P0.05)。DNMT1蛋白表达与组织分化类型、有无淋巴结转移及TNM临床分期相关(P0.05),与性别、年龄无关;而PDCD4蛋白的表达则只与区域淋巴结有无转移和TNM分期有关,与性别、年龄及组织病理分化程度无关。结论提示DNMT1、PDCD4蛋白异常表达与TSCC的发生、发展密切相关,DNMT1可能通过负向调节PDCD4的蛋白表达使抑癌基因沉默或消失而致肿瘤发生。
[Abstract]:Objective to investigate the expression and clinical significance of DNA methyltransferase (DNA) methyltransferase 1 (DNMT1) and programmed cell death factor (PDCD4) in squamous cell carcinoma of tongue (TSCC). Methods Immunohistochemical Elivision two-step method was used to detect 40 cases of TSCC and its corresponding paracancerous tissues. The expression of DNMT1pPDCD4 protein in non-tumor tissues, The relationship between the expression of DNMT1 protein and clinicopathologic parameters was analyzed. Results the expression of DNMT1 protein was high in TSCC, but the expression of PDCD4 protein was low or not. The expression of both proteins was negatively correlated with the type of tissue differentiation, lymph node metastasis and clinical stage of TNM, but had no relation with sex and age. However, the expression of PDCD4 protein was only related to regional lymph node metastasis and TNM staging, but not to sex, age and histological differentiation. Conclusion the abnormal expression of PDCD4 protein in DNMT1 was associated with the occurrence of TSCC. The development of DNMT1 may be associated with the silencing or disappearance of tumor suppressor genes by negatively regulating the protein expression of PDCD4.
【作者单位】：南昌大学第二附属医院口腔科;中国人民解放军第180医院烧伤整形科;广东医学院检验学院;
【分类号】：R739.86

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