长链非编码RNA在舌鳞癌中的表达谱特征

发布时间：2018-03-07 19:00

本文选题：长链非编码RNA　切入点：舌鳞癌　出处：《中山大学学报(医学科学版)》2014年03期 　论文类型：期刊论文

【摘要】：【目的】观察长链非编码RNA(lncRNA)在正常舌组织和舌鳞癌组织中表达谱的变化。【方法】利用lncRNA表达谱芯片检测技术,筛查3例舌鳞癌组织及3例正常舌组织中lncRNA表达谱的变异,通过对原始数据进行预处理达到均一化后,确定舌鳞癌组织中2倍以上变化并有显著差异(P0.05)的lncRNA为差异表达lncRNA,筛选出差异表达的lncRNA;分别在scc-9、cal-27、um-1和um-2共4种人舌鳞癌细胞和1种人正常舌细胞以及在35例舌鳞癌组织和12例正常舌组织中,利用实时荧光定量逆转录-多聚酶链反应(qRT-PCR)对芯片结果进行验证;对筛选所得的部分差异表达的lncRNA,利用lncRNA与mRNA特异性表达的标准化信号强度,构建基因共表达网络,并进行分析。【结果】芯片共筛选差异表达lncRNA 3590条,其中上调的共1785条,降低的共1805条;选取其中部分lncRNA,qRT-PCR进一步证实了芯片结果的准确性;共表达网络构建提示某些lncRNA可能在参与舌鳞癌发生发展过程中发挥重要调控作用。【结论】与正常舌组织比较,舌鳞癌组织lncRNA表达谱发生显著变化,提示差异表达的lncRNA可能与舌鳞癌的多种恶性表型有着密切联系。
[Abstract]:[objective] to observe the changes of lncRNA expression profile in normal tongue tissues and tongue squamous cell carcinoma tissues. [methods] the variation of lncRNA expression profile in 3 cases of tongue squamous cell carcinoma and 3 cases of normal tongue tissues was screened by lncRNA microarray. After preprocessing the raw data to achieve homogenization, LncRNA was identified as differentially expressed lncRNAs in tongue squamous cell carcinoma tissues with significant difference (P0.05), four human tongue squamous cell carcinoma cells and one human normal tongue cell line and 35 cases of tongue squamous cell carcinoma cell line scc-9 cal-27um-1 and um-2, respectively, were screened out for differential expression of LncRNAs. Tissue and 12 normal tongue tissues, Real-time fluorescence quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to verify the results of the microarray, and a gene coexpression network was constructed by using the standardized signal intensity of specific expression of lncRNA and mRNA to screen some differentially expressed LNC RNAs. [results] A total of 3590 differentially expressed lncRNA were screened on the chip, of which 1 785 were up-regulated and 1805 were decreased, and some of them were selected to confirm the accuracy of the results. The construction of coexpression network suggests that some lncRNA may play an important role in the development of tongue squamous cell carcinoma. [conclusion] compared with the normal tongue tissue, the expression profile of lncRNA in tongue squamous cell carcinoma is significantly changed. These results suggest that the differential expression of lncRNA may be closely related to the malignant phenotypes of tongue squamous cell carcinoma.
【作者单位】：南方医科大学附属口腔医院;广东省口腔医院口腔颌面外科;
【基金】：广东省级财政技术研究开发与推广应用专项资金(2013年) 广州市科技计划项目科技惠民专项
【分类号】：R739.8

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