硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制研究

发布时间：2018-03-08 19:03

本文选题：硅酸二钙　切入点：磷酸三钙　出处：《口腔医学研究》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制。方法:选取小鼠巨噬细胞系RAW264.7作为研究对象,磷酸三钙(Tricalcium phosphate,简称TCP)颗粒作为对比,硅酸二钙(Dicalcium silicate,简称C2S)、磷酸三钙颗粒均设置两种浓度:10mg/L和100 mg/L,观察RAW264.7对两种材料的吞噬作用,观察是否有细胞自噬现象发生,采用qRT-PCR的方法,检测硅酸二钙、磷酸三钙颗粒分别与RAW264.7共培养6h和24h后TLR2(Toll like receptor2)、TLR9基因的表达量,初步探讨硅酸二钙促炎反应的机制。结果:硅酸二钙与磷酸三钙颗粒与RAW264.7共培养后,细胞吞噬颗粒,通过透射电镜观察可见细胞自噬现象。与磷酸三钙颗粒组相比,硅酸二钙颗粒组与RAW264.7共培养6h后,TLR2、TLR9基因的表达均无明显变化;共培养24h后,硅酸二钙颗粒组的TLR2基因表达量明显增高(P0.001),10 mg/L磷酸三钙颗粒组的TLR2基因表达可能增高(0.05P0.1)。结论:硅酸二钙对小鼠巨噬细胞RAW264.7的促炎反应与TLR2基因的高表达可能有密切的关系。
[Abstract]:Objective: to investigate the mechanism of dicalcium silicate on the inflammatory response of mouse macrophage cell line RAW264.7. Methods: the mouse macrophage cell line RAW264.7 was selected as the study object and tricalcium phosphate (TCP) granules were used as control. Dicalcium silicate (C2SN) and tricalcium phosphate (tricalcium phosphate) particles were divided into two concentrations: 10 mg / L and 100 mg / L respectively. The phagocytosis of RAW264.7 on the two materials was observed, and the occurrence of autophagy was observed. Dicalcium silicate was detected by qRT-PCR. Tricalcium phosphate granules were co-cultured with RAW264.7 for 6 h and 24 h, respectively. The expression of TLR9 gene in TLR2(Toll like receptor 2 was studied. Results: after co-culture of dicalcium silicate and tricalcium phosphate with RAW264.7, the phagocytic granules were phagocytized. Compared with tricalcium phosphate granule group, the expression of TLR2TLR9 gene in dicalcium silicate group and RAW264.7 co-cultured for 6 h showed no significant change, but the expression of TLR9 gene in dicalcium silicate group was similar to that in tricalcium phosphate group after 24 hours of co-culture. The expression of TLR2 gene in dicalcium silicate group was significantly higher than that in the control group. The expression of TLR2 gene in tricalcium phosphate group may be increased by 0.05P0.10.Conclusion: the pro-inflammatory response of dicalcium silicate to RAW264.7 in mouse macrophages may be closely related to the high expression of TLR2 gene.
【作者单位】：广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室;
【基金】：广东省科学技术厅项目(编号:2016ZC0147);广东省科学技术厅项目(编号:2015110-13) 广州市荔湾区科技计划项目(编号:20151217094) 广州医科大学校级博士启动基金(编号:2015C43)
【分类号】：R783.1

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