不同牙周治疗手段对根面结构及牙周膜细胞生长的影响

发布时间：2018-03-09 00:04

本文选题：牙周炎　切入点：Nd　出处：《牙体牙髓牙周病学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的:观察评价离体条件下不同处理方式对牙周病患牙根面粗糙度及牙周膜细胞(PDLCs)在其表面粘附、增殖的影响。方法:将因重度慢性牙周炎而拔除的前磨牙和磨牙57个随机分为3组(n=19),分别用Gracey刮治器单纯手工刮治、手工刮治结合激光照射、手工刮治结合龈下喷砂的方法处理根面,用扫描电镜(SEM)观察表面形态、原子力显微镜(AFM)观察表面粗糙度后,将hPDLC接种于各组牙根片表面进行培养;分别于接种后1、6、12 h用SEM观察细胞粘附情况;培养后1、3、5 d MTT法检测细胞增殖情况。结果:SEM观察显示:Gracey组表面玷污层较多,存在器械划痕,激光组居中喷砂组最少;AFM观察显示:表面粗糙度以喷砂组最低,激光组最大(P0.05);牙周膜细胞接种于根面6 h后激光组与喷砂组细胞伸展充分,Gracey组细胞仍呈皱缩状,12 h后3组细胞均充分伸展;细胞接种于根面1、3、5 d后,各组的细胞数量均随培养时间而增加,激光组增殖最明显,喷砂组次之,Gracey组最低,各时间点各组间两两相比P0.05。结论:与单纯Gracey刮治相比,刮治结合激光照射、喷砂处理更有利于牙周膜细胞在根面的附着和增殖。
[Abstract]:Objective: to observe and evaluate the effect of different treatments on the surface adhesion of periodontal ligament cells (PDLCs) and the roughness of the root surface of periodontal disease in vitro. Methods: Fifty-seven premolars and molars extracted due to severe chronic periodontitis were randomly divided into 3 groups. The premolars and molars were treated with Gracey curette by hand and laser irradiation respectively. The root surface was treated by manual scraping combined with subgingival sand blasting. The surface morphology was observed by scanning electron microscope (SEM) and the surface roughness was observed by atomic force microscope (AFM). HPDLC was inoculated on the root surface of each group for culture. Cell adhesion was observed by SEM for 12 h after inoculation and proliferation was detected by MTT assay at 1: 3d after inoculation. Results the results showed that there were more smear layers and scratches on the surface of the cells in the group 1: Gracey. The results of AFM observation showed that the surface roughness of the laser group was the lowest in the sand blasting group. The cells of the laser group and the gracey group were all fully stretched 12 h after inoculation on the root surface 6 h after inoculation of the periodontal ligament cells, all the cells in the three groups were fully expanded after inoculation on the root surface for 3 days, and the cells in the Gracey group were still crumpled at 12 h after inoculation on the root surface. The number of cells in each group increased with culture time, the proliferation was most obvious in laser group, and the lowest in sand blasting group, P0.05.Conclusion: compared with simple Gracey curettage, curettage combined with laser irradiation can be used. Sand blasting is more conducive to the attachment and proliferation of periodontal ligament cells on the root surface.
【作者单位】：第四军医大学口腔医院牙周科军事口腔医学国家重点实验室;
【基金】：国家自然科学基金资助项目(81271137)
【分类号】：R781.4

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