不同生长因子对脂肪干细胞生物学行为的影响

发布时间：2018-03-09 22:24

本文选题：脂肪组织　切入点：干细胞　出处：《中国组织工程研究》2015年19期 　论文类型：期刊论文

【摘要】：背景:骨量不足限制了口腔种植修复的广泛应用,如何促进干细胞的迁移、黏附和增殖进而促进内源骨再生成为研究的关键点。目的:观察不同生长因子对体外培养兔脂肪干细胞迁移、黏附和增殖的影响,筛选出最佳的组合因子。方法:无菌切除兔腹股沟处的白色脂肪组织,采用酶消化法培养脂肪干细胞,取第3代细胞分为5组进行干预,分别为转化生长因子β1+血小板源性生长因子AB组(组1),血小板源性生长因子AB+血管内皮生长因子组(组2),转化生长因子β1+血管内皮生长因子组(组3),转化生长因子β1+血小板源性生长因子AB+血管内皮生长因子组(组4)和空白对照组。采用Transwell小室法检测细胞的迁移能力,黏附实验检测细胞的黏附能力,CCK-8法检测细胞的增殖能力。结果与结论:Transwell实验显示,4组间迁移细胞数差异有显著性意义(P0.05),其中,转化生长因子β1(2μg/L)+血小板源性生长因子AB(10μg/L)+血管内皮生长因子(10μg/L)组(组4)迁移细胞数最多,显著促进了脂肪干细胞的迁移。黏附实验结果显示,4组间黏附细胞数差异有显著性意义(P0.05),其中转化生长因子β1+血管内皮生长因子组(组3)黏附细胞数最多,显著促进了脂肪干细胞的黏附。CCK-8结果显示,在不同的时间点(1,3,5,7 d),各因子组合组吸光度值较对照组均显著增高(P0.05),其中血小板源性生长因子AB+血管内皮生长因子组(组2)递增的吸光度值最大,显著促进了兔脂肪干细胞的增殖。
[Abstract]:Background: insufficient bone mass limits the widespread application of dental implant repair, and how to promote the migration of stem cells. Adhesion and proliferation to promote endogenous bone regeneration become the key point of the study. Objective: to observe the effects of different growth factors on the migration, adhesion and proliferation of rabbit adipose stem cells in vitro. Methods: the white adipose tissue of rabbit groin was excised aseptically, the adipose stem cells were cultured by enzyme digestion, and the third passage cells were divided into 5 groups for intervention. TGF- 尾 1 platelet-derived growth factor AB group (group 1), platelet derived growth factor group AB vascular endothelial growth factor group (group 2), transforming growth factor 尾 1 group (group 3), transforming growth factor group (group 3). Platelet derived growth factor AB (group 4) and blank control group (group 4) were used to detect the migration ability of cells by Transwell chamber assay. The ability of cell adhesion was detected by cell adhesion assay and the cell proliferation was detected by CCK-8 method. Results and conclusion the number of migrating cells in the four groups was significantly different from that in the control group (P 0.05). Transforming growth factor 尾 1 2 渭 g / L) platelet-derived growth factor (AB(10 渭 g / L) vascular endothelial growth factor 10 渭 g / L (group 4) had the highest number of migrating cells, The adhesion experiment showed that there was significant difference in the number of adhesion cells among the four groups, especially in the transforming growth factor 尾 1 vascular endothelial growth factor group (group 3). Significantly increased the adhesion of adipose stem cells. CCK-8 results showed that, At different time points, the absorbance value of each factor combination group was significantly higher than that of the control group (P 0.05), and the increasing absorbance value of platelet derived growth factor AB vascular endothelial growth factor (group 2) was the highest. It significantly promoted the proliferation of rabbit adipose stem cells.
【作者单位】：潍坊医学院口腔医学院;济南市中心医院口腔科;济南市市中区人民医院口腔科;
【基金】：山东省科技发展项目(2014GSF11804)~~
【分类号】：R783

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