3D打印明胶海藻酸钠凝胶支架对人牙髓细胞的黏附增殖作用

发布时间：2018-03-11 13:35

本文选题：D打印明胶海藻酸钠　切入点：人牙髓细胞　出处：《南方医科大学学报》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨3D打印的明胶海藻酸钠凝胶支架对人牙髓细胞(HDPCs)的细胞毒性,及不同接种方法对细胞黏附生长的差异。方法组织块酶消化法分离培养HDPCs,利用Bioplotter三维生物打印机进行明胶海藻酸钠凝胶支架的3D打印,选取第3代HDPCs,Cell Counting Kit-8试剂检测不同浓度材料浸提液对细胞增殖的影响,扫描电镜观察、台盼蓝染色计数比较直接滴加低浓度细胞悬液与高浓度细胞浓缩液接种法,对细胞在材料表面黏附与增殖作用的差异。结果不同浓度的材料浸提液均对HDPCs细胞毒性为0,具有促进细胞增殖的作用。HDPCs在3D打印的明胶海藻酸钠凝胶支架上生长良好,先滴加高浓度的细胞浓缩液可以更有效的促进细胞对材料的黏附,5d后材料表面的细胞计数结果较滴加低浓度细胞悬液显著增加,差异具有统计学意义(P0.05)。结论 3D打印的明胶海藻酸钠凝胶支架具有良好的生物相容性,具有促进HDPCs增殖的作用,可作为牙再生的支架材料,选择高浓度细胞浓缩液接种细胞更有利于细胞黏附。
[Abstract]:Objective to investigate the cytotoxicity of 3D printed gelatin sodium alginate gel scaffold on human dental pulp cells (HDPCs). Methods HDPCs were isolated and cultured by tissue mass enzyme digestion, and 3D printing of gelatin sodium alginate gel scaffold was carried out by Bioplotter three-dimensional bioprinter. The third generation of HDPCS cell Counting Kit-8 reagent was selected to detect the effect of extracts of different concentration materials on cell proliferation. Scanning electron microscope (SEM) was used to observe the cell proliferation. Trypan blue staining was compared with the method of directly adding low concentration cell suspension and high concentration cell concentrate. Results the toxicity of different concentrations of extractions to HDPCs cells was 0. HDPCs could promote cell proliferation. HDPCs grew well on 3D printed gelatin sodium alginate gel scaffold. After 5 days of cell adhesion, the cell count on the surface of the material was significantly increased compared with that of the cell suspension added with low concentration. Conclusion the gelatin sodium alginate gel scaffold with 3D printing has good biocompatibility and can promote the proliferation of HDPCs, so it can be used as a scaffold for tooth regeneration. High concentration of cell concentrate was more favorable for cell adhesion.
【作者单位】：南方医科大学南方医院;南方医科大学口腔医学院;
【基金】：国家自然科学基金青年项目(81400495) 广东省自然科学基金博士启动项目(2015A3030310101)~~
【分类号】：R783.1

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