Th17细胞在人慢性根尖周炎中的定位研究

发布时间：2018-03-12 14:03

本文选题：根尖肉芽肿　切入点：根尖囊肿　出处：《安徽医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的通过检测Th17细胞的特异性标记维甲酸相关孤核受体(Retinoic acid-relatedorphan nuclear receptor, ROR)-γT和白细胞介素(interleukin, IL)-17在人根尖肉芽肿和根尖囊肿中的表达，来阐明Th17细胞在根尖周病变中的存在情况及作用。方法收集安徽医科大学附属口腔医院口腔颌面外科2011年11月至2013年7月经根尖手术切除的根尖周炎病损组织，将其进行病理鉴定选取根尖肉芽肿和根尖囊肿组织作为实验组，同时进行炎症分级。收集埋伏阻生智齿拔除时需凿骨患者的健康牙槽骨5例，作为正常对照组。免疫组织化学分析所有样本中RORγT和IL-17的表达情况。采用Image Pro Plus6.0对所有样本中RORγT和IL-17的蛋白表达量进行检测。结果共有根尖囊肿18例，根尖肉芽肿22例，其中，轻度炎症浸润5例，中度炎症浸润11例，重度炎症浸润24例。RORγT表达于所有的根尖周炎病损组织中并显著高于正常对照组(P＜0.05)和阴性对照组(P＜0.05)，正常对照组和阴性对照组中没有检测到RORγT的表达，但其在根尖肉芽肿和根尖囊肿中的表达无显著差异(P=0.967)；RORγT在轻度、中度和重度炎症细胞浸润中的表达水平均显著高于正常对照组和阴性对照组(P＜0.05)，但三组间并无统计学差异；与对照组相比，IL-17在根尖周炎病损组织中的的蛋白表达水平均显著增高。RORγT和IL-17的蛋白表达水平在根尖肉芽肿和根尖囊肿中均呈正相关，分别为r=0.935，P＜0.05和r=0.803，P＜0.05。结论 1.本实验通过免疫组织化学的方法，借助Th17细胞的特异性标记RORγT和IL-17，初步证明了Th17细胞存在于人根尖肉芽肿和根尖囊肿的病损组织中。 2. RORγT和IL-17在正常根尖周组织中没有表达，提示Th17细胞可能参与了根尖周病的发病过程。 3. RORγT和IL-17在不同炎症浸润程度之间的差异并无统计学意义，提示Th17细胞可能对根尖周病变的炎症进展作用不大。
[Abstract]:Objective to detect the expression of Retinoic acid-relatedorphan nuclear receptor, ROR- 纬 T and interleukin, IL)-17 in human apical granuloma and apical cyst by the specific labeling of retinoic acid acid-relatedorphan nuclear receptor in Th17 cells. To elucidate the existence and role of Th17 cells in periapical lesions. Methods the lesions of periapical periodontitis were collected from November 2011 to 2013 in oral and maxillofacial surgery, affiliated stomatology hospital of Anhui Medical University. The apical granuloma and apical cyst tissue were selected as experimental group and inflammatory grading was performed. 5 cases of healthy alveolar bone were collected from patients with impacted impacted wisdom teeth. The expression of ROR 纬 T and IL-17 in all samples was analyzed by immunohistochemistry as a normal control. Image Pro Plus6.0 was used to detect the protein expression of ROR 纬 T and IL-17 in all samples. Results there were 18 cases of apical cyst and 22 cases of apical granuloma. The expression of .ROR 纬 T in 24 patients with severe inflammatory infiltration was significantly higher than that in normal control group (P < 0.05) and negative control group (P < 0.05), but no expression of ROR 纬 T was detected in normal control group and negative control group. However, there was no significant difference in the expression of ROR 纬 T between apical granuloma and apical cyst. The expression of ROR 纬 T in mild, moderate and severe inflammatory cell infiltration was significantly higher than that in normal control group and negative control group (P < 0.05), but there was no significant difference among the three groups. Compared with the control group, the expression of IL-17 protein in periapical lesions was significantly higher. ROR 纬 T and IL-17 protein expression levels were positively correlated with those in apical granuloma and apical cysts, which were respectively 0. 05 and 0. 803 (r = 0. 05) and 0. 803 (P < 0. 05), respectively. Conclusion. 1. In this study, the presence of Th17 cells in the diseased tissues of human apical granuloma and apical cyst was preliminarily demonstrated by the immunohistochemical method and the specific labeling of ROR 纬 T and IL-17 by Th17 cells. 2. ROR 纬 T and IL-17 were not expressed in normal periapical tissues, suggesting that Th17 cells may be involved in the pathogenesis of periapical disease. 3. There was no significant difference between ROR 纬 T and IL-17 in different degree of inflammatory infiltration, suggesting that Th17 cells may have little effect on inflammatory progression of periapical lesions.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.3

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