磁力矫治器模拟静磁场对SD大鼠面颌肌细胞增殖及细胞周期的影响

发布时间：2018-03-14 07:46

本文选题：面颌肌细胞　切入点：静磁场　出处：《实用口腔医学杂志》2014年01期 　论文类型：期刊论文

【摘要】：目的:研究磁力矫治器产生静磁场对SD大鼠面颌肌细胞增殖及细胞周期的影响。方法:将体外培养的第3代面颌肌细胞实验组加载磁场强度为971.77 mT的静磁场,对照组无磁场处理。用酶联免疫检测仪和流式细胞仪测定培养48 h和72 h后各组细胞的吸光度(A)值和细胞周期,并对其结果进行统计学分析。结果:实验组A值明显高于对照组(P0.05)。细胞周期结果显示实验组G0/G1期比例比对照组低(P0.05),S期和G2/M期细胞比例、PI值均比对照组高(P0.05)。结论:磁力矫治器静磁场有促进面颌肌细胞增殖的作用,促进细胞由G0/G1期向S期、S期向G2/M期转化。
[Abstract]:Objective: to study the effect of magnetostatic field produced by magnetic appliance on proliferation and cell cycle of facial muscle cells in SD rats. Methods: the experimental group of the third generation of facial maxillary muscle cells in vitro was subjected to magnetostatic magnetic field of 971.77 Mt. In the control group, the absorbance of cells and cell cycle were measured by enzyme-linked immunosorbent assay (Elisa) and flow cytometry (FCM) after 48 and 72 hours of culture. The results were statistically analyzed. Results: the value of A in the experimental group was significantly higher than that in the control group (P 0.05). The cell cycle results showed that the ratio of G 0 / G 1 phase in the experimental group was lower than that in the control group (P 0.05) and the Pi value in the G 2 / M phase was significantly higher than that in the control group (P 0.05). The magnetostatic magnetic field can promote the proliferation of facial muscle cells. Promote cell transformation from G _ 0 / G _ 1 phase to S phase S phase to G _ 2 / M phase.
【作者单位】：齐齐哈尔医学院第五附属医院;大庆龙南医院口腔科;昆明医科大学附属口腔医院口腔正畸科;
【基金】：国家自然科学基金(编号:30700964,81260162)
【分类号】：R783

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