Micro-CT重建时分析区域变化对颅顶骨骨量参数的影响

发布时间：2018-03-15 21:12

本文选题：显微CT　切入点：分析区域　出处：《重庆医科大学学报》2017年09期 　论文类型：期刊论文

【摘要】：目的:在分析区域包含整个颅顶骨的前提下,改变颅顶骨显微CT(micro-computed tomography,Micro-CT)扫描后二维图像的重建层数或者位置,比较进行重建后的骨量参数(骨表面积、骨体积)是否出现改变。方法:4日龄小鼠颅顶骨培养后置于含培养液的EP管中,进行连续Micro-CT扫描。根据重建时改变层数或位置来分别分析:(1)996~22层组与800~50层组,分析层数不同(n=3);(2)996~22层组与随机选择层组,分析层数不同(n=3);(3)近边界上缘组与近边界下缘组,分析层数相同(n=3)的骨量参数变化。结果:在分析区域包含整个颅顶骨的前提下:(1)增加或者减少层数导致骨表面积变化(F=47.798,P=0.002),而骨体积无变化(F=7.083,P=0.056)。(2)随机选择层数组与996~22层数组分析比较时骨表面积(F=359.392,P=0.000)、骨体积(F=51.844,P=0.002)均有变化。(3)重建层数相同的前提下,改变分析区域位置时骨表面积(F=0.000,P=0.991)、骨体积(F=0.000,P=0.995)无变化。结论:(1)Micro-CT三维重建时,改变重建层数可影响骨量参数。(2)Micro-CT三维重建时,随机选择重建层数可影响骨量参数。(3)Micro-CT三维重建时,保证分析层数相同而改变空白分析区域的位置不会影响骨量参数。
[Abstract]:Objective: to change the number or position of reconstruction layers in the two-dimensional images of the parietal bone after scanning, and to compare the bone mass parameters (bone surface area) after the reconstruction by analyzing the region including the whole cranio-parietal bone. Methods the cranio-parietal bone was cultured in EP tube containing culture medium and continuous Micro-CT scanning was performed. According to the number or position of the layers changed during reconstruction, the two groups were analyzed respectively. Different layers are analyzed, such as the formation of layer 22 and the group of randomly selected layers. The number of layers is different, the upper and lower edge groups of the near boundary are different, and the formation of the upper edge of the near boundary and the group of the lower edge of the near boundary are analyzed. Results: under the condition that the analytical region contains the whole cranio-parietal bone, the increase or decrease of the number of layers leads to the change of bone surface area. When the bone surface area is 359.392U 0.000g, the bone volume is 51.844P0. 002) the number of the reconstructed layers is the same, and the number of the reconstructed layers is the same when the bone surface area is 359.392g, and the bone volume is 51.844p0. 002). There was no change in bone surface area and bone volume when the position of the analyzed area was changed. Conclusion in the 3-D reconstruction of micro-CT, changing the number of reconstructed slices can affect the bone mass parameters. The random selection of the reconstructed slice number can affect the three-dimensional reconstruction parameters of .3micro-CT. Make sure that the number of layers is the same and change the position of blank analysis area will not affect the bone mass parameters.
【作者单位】：重庆医科大学附属口腔医院修复科;
【基金】：国家自然基金青年科学基金资助项目(编号:81200767/H1402)
【分类号】：R782

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