叶酸壳聚糖载地西他滨纳米粒的制备及其对SCC-9细胞的抑制作用

发布时间：2018-03-17 01:17

本文选题：叶酸　切入点：壳聚糖　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:合成性质稳定的叶酸壳聚糖载地西他滨纳米粒,观察其对体外培养人鳞状上皮舌癌SCC-9细胞的抑制率,探讨纳米粒对肿瘤细胞的靶向作用机制。方法:采用离子交联法合成叶酸壳聚糖载地西他滨纳米粒,透射电镜观察其形态,纳米粒度分析仪测量纳米粒平均粒径、电位,检测载药率以及包封率。将体外培养的处于对数期的人鳞状上皮舌癌SCC-9细胞分为0、1、2、3、4、5、6、7八组。1、2、3组选用三个梯度浓度1×l0-7mol/L、1×l0-6mol/L、1×l0-5mol/L的DAC处理细胞,4、5、6组采用同样三个浓度梯度1×l0-7mol/L、1×l0-6 mol/L、1×l0-5mol/L的叶酸壳聚糖载地西他滨纳米粒处理细胞,7组为叶酸壳聚糖空载体组,0组为空白对照组。MTT法检测各组对舌癌细胞的抑制率。结果:叶酸壳聚糖载地西他滨纳米粒形态为球形,分布均匀,平均粒径为212nm,Zeta电位绝对值平均值为31.8mv,性质稳定。载药纳米粒的包封率为75%,载药率为18%。叶酸壳聚糖载地西他滨纳米粒组较单纯地西他滨组对人鳞状上皮舌癌SCC-9细胞抑制率高,且差异具有统计学意义。结论:(1)本实验成功合成叶酸壳聚糖载地西他滨纳米粒;(2)叶酸壳聚糖载地西他滨纳米粒性质稳定,载药率以及包封率较高;(3)叶酸壳聚糖载地西他滨纳米粒可能靶向作用于人鳞状上皮舌癌SCC-9细胞,抑制SCC-9的生长。
[Abstract]:Aim: to synthesize stable chitosan-loaded dicitabine nanoparticles and observe its inhibitory rate on human squamous cell carcinoma of tongue (SCC-9) cells in vitro. Methods: chitosan folate loaded dicitabine nanoparticles were synthesized by ion crosslinking method. The morphology of nanoparticles was observed by transmission electron microscope. The average particle size and potential of nanoparticles were measured by nano-particle size analyzer. The SCC-9 cells in logarithmic phase of human squamous cell carcinoma of tongue were divided into three groups: 1 脳 l0-7 mol / L 1 脳 l0-6 mol / L 1 脳 l0-6 mol / L 1 脳 l0-6 mol / L DAC with the same concentration gradient 1 脳 l0-7 mol / L 1 脳 l0-6 mol / L ~ (-1) 脳 l0-5 mol / L ~ (-1) 脳 l0-5 mol / L ~ (-1). The inhibitory rate of folate chitosan loaded dietabine nanoparticles on tongue cancer cells was detected by MTT method. Results: the morphology of the nanoparticles was spherical. Evenly distributed, The mean particle size was 212nmPe, the mean absolute value of Zeta potential was 31.8mv.The entrapment efficiency of drug loaded nanoparticles was 75 and the drug loading rate was 18.The inhibitory rate of dietabine nanoparticles loaded with folate chitosan on SCC-9 cells of human squamous epithelial tongue carcinoma was higher than that of dietabine group. ConclusionChitosan-loaded dietabine nanoparticles containing folate chitosan were successfully synthesized in this experiment. The drug loading rate and encapsulation efficiency were higher than that of the control group) chitosan / dicitabine nanoparticles might target SCC-9 cells and inhibit the growth of SCC-9 in human squamous cell carcinoma of tongue.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.86

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