口腔鳞状细胞癌中STING的表达及意义

发布时间：2018-03-17 22:34

本文选题：口腔肿瘤　切入点：鳞状细胞癌　出处：《临床与实验病理学杂志》2014年07期 　论文类型：期刊论文

【摘要】：目的探讨干扰素刺激基因(stimulator of interferon genes,STING)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织及其对应癌旁组织中的表达及其临床诊断意义。方法收集25例OSCC组织及对应癌旁组织,提取组织内mRNA,采用SYBR Green荧光实时定量PCR技术检测组织中STING、TBK-1和IRF-3的表达,并分析正常角朊细胞系和OSCC细胞系中IRF-3的表达。结果 OSCC组织内STING的表达与对应癌旁组织相比显著下降(P=0.014 1);OSCC组织内STING下游基因TBK-1的表达与对应癌旁组织相比显著下降(P=0.030 6);OSCC组织内IRF-3的表达与对应癌旁组织相比显著下降(P=0.0313);OSCC细胞系HSC-3与正常细胞系HaCaT细胞系相比,IRF-3 mRNA表达显著降低(P=0.009 3)。结论 STING及其下游基因在OSCC癌灶组织内低表达,下调的STING-TBK-1-IRF-3通路可能与癌症进程有关。
[Abstract]:Objective to investigate the expression of interferon stimulator of interferon geneshiza (STINGA) in oral squamous cell carcinoma (OSCC) and its clinical diagnostic significance. Methods 25 cases of OSCC and corresponding adjacent tissues were collected. The expression of STINGTBK-1 and IRF-3 in tissues was detected by SYBR Green real-time quantitative PCR. The expression of IRF-3 in normal keratinocytes and OSCC cell lines was analyzed. Results the expression of STING downstream gene TBK-1 in OSCC tissues was significantly lower than that in corresponding paracancerous tissues. The expression of IRF-3 in OSCC was significantly lower than that in the corresponding adjacent tissues. The expression of IRF-3 mRNA in the HSC-3 cell line was significantly lower than that in the normal cell line HaCaT cell line. Conclusion the expression of STING and its downstream genes are low in the tissues of OSCC carcinomas. The down-regulated STING-TBK-1-IRF-3 pathway may be associated with cancer progression.
【作者单位】：南京大学口腔医学院;南京市口腔医院口腔病理科;南京市口腔医院中心实验室;江苏省医学分子技术重点实验室;
【基金】：国家自然科学基金(81072213、81271698) 国家临床重点专科建设项目(2011) 江苏省临床医学科技专项项目(BL2012017) 南京市医学重点实验室建设项目(2012) 南京市医学科技发展项目(YKK09124、YKK13145) 江苏省卫生厅面上科研课题(H201344) 南京卫生青年人才(QRX113311)
【分类号】：R739.8

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