前导改变对大鼠颞下颌关节滑膜中iNOS及COX-2表达影响的研究

发布时间：2018-03-18 00:29

本文选题：前导斜度　切入点：动物模型　出处：《口腔医学研究》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨实验性前导斜度改变对大鼠颞下颌关节(temporomandibular joint,TMJ)滑膜中诱导型一氧化氮合成酶(iNOS)及环氧合酶-2(COX-2)表达的影响。方法:采用低速金刚砂轮打磨大鼠上下前牙,建立前导缺失模型。在大鼠上前牙粘结舌侧增大15°、30°金属冠,建立相应前导增大15°、30°模型;3、7、14、28d每组各取2只大鼠一侧TMJ及另一侧TMJ滑膜组织,免疫组织化学染色(immunohistochemistry,IHC)及实时定量聚合酶链反应(Real-time polymerase chain reaction,Real-time PCR)检测TMJ滑膜iNOS和COX-2的表达情况。结果:前导斜度增大30°组COX-2、iNOS表达较对照组显著增强(P0.008)。其中COX-2在3d时表达最高,3、7d较28d有统计学差异(P0.008)。iNOS在7d时表达最高,较3、14、28d表达无统计学差异。结论:iNOS及COX-2可能是前导异常造成颞下颌关节滑膜炎的重要分子机制之一。
[Abstract]:Objective: To investigate the leading inclination on the temporomandibular joints of the rats (temporomandibular, joint, TMJ) inducible nitric oxide synthase in the synovium (iNOS) and cyclooxygenase (COX-2) on the expression of -2. Methods: Using low speed diamond grinding wheel in upper and lower anterior teeth, anterior teeth missing. A leading model of adhesive tongue side increases 15 degrees in rats, 30 degrees of metal crown, establish the corresponding leading increased 15 degree, 30 degree model; 3,7,14,28d group 2 rats from each side of the TMJ and the other side of the TMJ synovial tissue, immunohistochemical staining (immunohistochemistry, IHC) and real-time quantitative polymerase chain reaction (Real-time polymerase chain reaction, Real-time PCR) to detect TMJ expression of iNOS and COX-2 in synovial membrane. Results: the leading angle increased 30 degree of COX-2 group, the expression of iNOS group increased significantly compared to the control (P0.008). The COX-2 3D expression in 3,7d was highest, 28d had significant difference (P0.008) .iNOS expression was highest at 7d, and no difference compared with 3,14,28d expression. Conclusion: iNOS and COX-2 may be one of the important molecular mechanisms of temporomandibular synovitis caused by abnormal precursor.

【作者单位】：陕西省颅颌面精准医学研究重点实验室;西安交通大学口腔医院口腔综合科;西安交通大学口腔医院口腔修复科;银川市口腔医院口腔修复科;
【基金】：陕西省社会发展科技攻关基金(编号:2012k16-10-03)
【分类号】：R782.6

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