牵张应变诱导人牙周膜细胞凋亡过程中caspase蛋白酶表达的初步研究

发布时间：2018-03-18 06:35

本文选题：人牙周膜细胞　切入点：牵张　出处：《医用生物力学》2017年02期 　论文类型：期刊论文

【摘要】：目的研究在牵张应变诱导人牙周膜细胞凋亡过程中caspase蛋白酶表达的变化。方法对体外培养的人牙周膜细胞分别施加20%牵张应变6、24 h,采用流式细胞术测定细胞凋亡率,采用免疫印迹技术检测caspase-3、-5、-7、-8、-9的蛋白表达,并采用分光光度比色法测定caspase-3、-5、-8、-9的蛋白酶活性变化。结果 20%牵张应变加载6、24 h可诱导人牙周膜细胞发生凋亡。caspase-3的蛋白表达及蛋白酶活性和caspase-7的蛋白表达在24 h牵张应变作用下较对照组增加,caspase-5、-8、-9的蛋白表达及蛋白酶活性在6、24 h牵张应变作用下较对照组增加。结论 20%牵张应变能诱导体外培养的人牙周膜细胞凋亡,此过程中伴随发生了caspase-3、-5、-7、-8、-9蛋白酶的活化。
[Abstract]:Objective to study the expression of caspase protease in human periodontal ligament cells induced by stretch strain. Methods 20% stretch strain was applied to cultured human periodontal ligament cells for 24 h and the apoptosis rate was measured by flow cytometry. Western blotting technique was used to detect the protein expression of caspase-3, caspase-3, caspase-3, caspase-3, caspase-3, caspase-3, caspase-3, caspase-3, The protease activity of caspase-3 and caspase-3 was determined by spectrophotometric colorimetry. Results the protein expression of caspase-3, protease activity and protein expression of caspase-7 in periodontal ligament cells were induced by 20% stretch strain loading for 6h for 24 h. Compared with the control group, the protein expression of caspase-5, caspase-5 and the activity of protease were increased under tensile strain. Conclusion 20% strain can induce apoptosis of human periodontal ligament cells in vitro. This process was accompanied by the activation of caspase-3-5-5-7-7-8-8-9 protease.
【作者单位】：上海交通大学医学院附属第九人民医院口腔修复科;上海市口腔医学研究所上海市口腔医学重点实验室;
【基金】：国家自然科学基金项目(31470903,31270991,30900282) 上海市浦江人才计划项目(13PJD021) 上海市青年科技启明星计划(A类)项目(10QA1404200) 上海市重点学科建设项目(T0202,S30206-sms02)
【分类号】：R781

【相似文献】