纳米羟基磷灰石表面阿伦膦酸钠和Ⅰ型胶原蛋白对成骨细胞形态和增殖的影响

发布时间：2018-03-19 12:27

  本文选题：纳米羟基磷灰石　切入点：阿伦膦酸钠I型胶原蛋白　出处：《福建医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：考察纳米羟基磷灰石（nano-hydroxyapatite，，nHA）涂层表面阿伦膦酸钠（alendronate sodium，AL）和I型胶原蛋白（collagen type I，COL I）对成骨细胞生长、黏附和增殖的影响。方法：采用电化学沉积技术在纯钛（pure titanium，PT）表面沉积nHA涂层，通过扫描电子显微镜（scanning electron microscope，SEM）、X射线能谱分析仪（energy dispersive X-ray spectroscopy，EDS）、X射线衍射仪（X-ray diffraction，XRD）和傅里叶红外光谱仪（fourier transform infraredspectroscopy，FTIR）表征。而后采用化学结合法在nHA涂层上制备nHA/AL、nHA/COL I、nHA/AL/COL I和nHA/COL I/AL复合膜，通过接触角测量仪（contactangle meter，CAM）、X射线光电子能谱仪（X-ray photoelectron spectrometer，XPS）和FTIR进行表征。在实验组：nHA/AL/COL I和nHA/COL I/AL复合膜及对照组：nHA涂层、nHA/AL和nHA/COL I复合膜表面培养成骨细胞，通过SEM观察成骨细胞形态，WST-1检测复合膜对成骨细胞生长和增殖的影响。结果：nHA涂层表面细胞增殖能力最低，与各复合膜之间的差异均有统计学意义（P0.05），nHA/COL I复合膜的增殖能力稍高于nHA/AL复合膜（P0.05），nHA/AL/COL I和nHA/COL I/AL复合膜的增殖活性均高于对照组（P0.05），但nHA/AL/COL I与nHA/COL I/AL复合膜的细胞增殖活性的差异无统计学意义（P0.05）。nHA/AL/COL I和nHA/COL I/AL复合膜表面成骨细胞充分伸展，多层细胞形成致密的板层样结构，nHA/AL和nHA/COL I复合膜表面细胞重叠生长，而nHA涂层表面细胞较少，可见大面积nHA基底。结论：10-6M AL和COL I均能促进成骨细胞的黏附和增殖，两者共同作用时作用更强。nHA表面AL和COL I的结合次序对成骨细胞的生长和增殖能力无明显影响。种植体表面AL和COL I的联合应用值得进一步研究探索。
[Abstract]:Objective: to investigate the effects of alendronate sodium alendronate (ALL) and collagen I collagen I (Col I) on the growth, adhesion and proliferation of osteoblasts on the surface of nano-hydroxyapatite nano-hydroxyapatite (NHA) coating. Scanning electron microscopy (SEM) and scanning electron microscopy (SEM) were used to characterize the electron microscopesimetry (SEM) and X-ray diffraction (dispersive) dispersive X-ray spectrometer (EDR) and Fourier transform infrared spectroscopy (FTIR). Then the composite films of nHAR / ALNHACOL / InHAP / COL and nHA/COL / IRA were prepared on nHA coating by chemical combination method. X-ray photoelectron spectrometerus XPSs and FTIR were used to characterize the osteoblasts on the surface of the experimental group and the control group. The osteoblasts were cultured on the surface of the NHA / all / Col / nHA/COL / IAL composite membrane and the control group (n / nHA coating / nHAAL / nHA/COL I composite membrane). The effect of the composite membrane on the growth and proliferation of osteoblasts was observed by SEM. The proliferative activity of P0.05 / Col I composite membrane was slightly higher than that of nHA/AL composite membrane P0.05 / all / Col I and nHA/COL I / AL composite membrane, but the cell proliferation activity of nHA/AL/COL I / nHA/COL I / AL composite membrane was higher than that of the control group. There was no statistically significant difference in the number of osteoblasts on the surface of P0.05nHA / all / Col I and nHA/COL / I / AL composite membranes. The multilayer cells formed a dense lamellar structure, the cells on the surface of NHA / AL and nHA/COL I composite membrane superposed, but there were few cells on the surface of nHA coating, which showed that there was a large area of nHA substrate. Conclusion\% 10 ~ (-6) M AL and COL I can promote the adhesion and proliferation of osteoblasts. The binding order of AL and COL I on the surface of. NHA has no significant effect on the growth and proliferation of osteoblasts. The combined application of AL and COL I on implant surface is worthy of further study.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.6


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