免疫组化双染技术观察大鼠萎缩腮腺中肌上皮细胞的增殖变化

发布时间：2018-03-19 15:47

本文选题：肌上皮细胞增殖　切入点：腮腺　出处：《实用口腔医学杂志》2014年03期 　论文类型：期刊论文

【摘要】：目的:观察大鼠腮腺主导管结扎后腺体萎缩及肌上皮细胞(myoepithelial cell,MEC)的增殖变化规律。方法:对78只Wistar大鼠结扎右侧腮腺主导管,HE染色观察正常腮腺及主导管结扎后0(对照)、1、3、5、7、14、21、30、60、90、120、150、180 d腮腺的组织学变化(n=6),免疫组化双重染色方法定量分析MEC在腮腺萎缩不同时间点的增殖率及分布情况。结果:主导管结扎后,腮腺腺泡细胞逐渐凋亡消失,大量导管样结构形成,纤维组织增生并伴炎性细胞浸润。导管结扎后第3天,MEC快速增殖,5 d时增殖率达到峰值,7 d后MEC增殖率降低,30~150 d时MEC增殖率维持在一个较低水平,180 d时未观察到增殖的MEC,MEC总数显著减少。结论:结扎腮腺导管诱导腺体萎缩的过程中MEC早期增殖,随后增殖活力下降、停止。
[Abstract]:Objective: to observe the changes of atrophy of glands and myoepithelial cells of myoepithelial cells (MECs) after ligation of main parotid duct in 78 Wistar rats. Methods: the normal parotid gland and the normal parotid duct were observed by HE staining after ligation of the right parotid duct in 78 Wistar rats. The histopathological changes of parotid gland were analyzed by immunohistochemical double staining method. The proliferative rate and distribution of MEC at different time points of parotid atrophy were analyzed by immunohistochemical double staining. Results: after ligation of main duct, the proliferation rate and distribution of MEC in parotid gland atrophy at different time points were analyzed. The apoptosis of acinar cells in parotid gland gradually disappeared and a large number of catheter-like structures were formed. The proliferative rate of MEC reached its peak at the 5th day after ligation of the catheter. The proliferative rate of MEC was decreased at 30 ~ 150 d. The proliferative rate of MEC was maintained at a relatively low level at 180 d after ligation of the catheter. Conclusion: the early proliferation of MEC in the process of gland atrophy induced by ligation of parotid ducts is a significant decrease in the total number of proliferative MECs. Then the proliferation activity decreased and stopped.
【作者单位】：滨州医学院附属医院口腔颌面外科;滨州医学院附属医院病理科;
【基金】：山东省医药卫生科技发展计划基金(编号:2011HW-004)
【分类号】：R781.7

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