富血小板纤维蛋白对人牙周膜成纤维细胞迁移及成骨分化的影响

发布时间：2018-03-20 11:42

本文选题：牙周膜成纤维细胞　切入点：富血小板纤维蛋白　出处：《郑州大学学报(医学版)》2014年01期 　论文类型：期刊论文

【摘要】：目的:观察Choukroun's富血小板纤维蛋白(PRF)对自体人牙周膜成纤维细胞(hPDLCs)迁移、成骨分化的影响,探讨PRF在牙周组织再生治疗中的潜能。方法:原代培养hPDLCs;制备PRF,实验分为P1组(1片PRF浸出液)、P2组(2片PRF浸出液)和对照组,划痕实验观察记录各组第24、48、72 h细胞迁移的距离;Transwell制备迁移模型,按实验分组培养24 h后结晶紫染色观察;成骨矿化诱导液制备不同浓度的PRF浸出液(P1组、P2组),碱性磷酸酶(ALP)试剂盒检测3、5、7 d细胞破碎液中ALP活性;成骨矿化诱导液连续培养21 d,茜素红染色后测定矿化结节面积。结果:划痕实验中P1组、P2组细胞迁移距离大于对照组(F=316.248、32.846和1 169.847,P均0.001),P1组、P2组比较差异无统计学意义(P均0.05)。Transwell迁移实验中P1组、P2组与对照组比较,迁移细胞数增加(F=742.729,P0.001),P1组、P2组比较差异无统计学意义(P0.05)。ALP活性P1组、P2组与对照组比较差异均有统计学意义(F=474.202、1 383.521、2 317.965,P均0.001),P1组、P2组比较差异无统计学意义(P均0.05)。P1组、P2组与对照组矿化结节面积比较差异有统计学意义(F=332.280,P0.001),P1组与P2组比较差异无统计学意义(P0.05)。结论:PRF对hPDLCs具有促进其迁移和成骨分化的作用,提示PRF在牙周组织再生工程中具有很大的临床应用潜能。
[Abstract]:Objective: to observe the effect of Choukroun's rich platelet fibrin on the migration and osteogenic differentiation of human periodontal ligament fibroblasts. To investigate the potential of PRF in periodontal tissue regeneration. Methods: the primary culture of hPDLCswas used to prepare PRF, which was divided into P1 group and control group. The migration model was established by Transwell after 24 ~ 48h 72 h cell migration was recorded by scratch test. Crystal violet staining was observed after 24 h culture. Different concentrations of PRF leaching solution were prepared by osteoblastic inducer. The ALP activity was detected by alkaline phosphatase (ALP) kit in group P _ 1 and B _ (2) group. The activity of ALP in the cell fragmentation solution was detected by alkaline phosphatase assay (ALP) kit for 7 days. The mineralized nodule area was determined by alizarin red staining for 21 days. Results: in the scratch test, the migration distance of P _ 1 group was higher than that of the control group (F _ (316.248A) 32.846 and P _ (1 169.847) P 0.001) P ~ (2) group. There was no significant difference in cell migration distance between P _ (2) group and P _ (2) group. In the transwell migration experiment, the P _ 1 group and P _ 2 group were compared with those in the control group. There was no significant difference in the number of migration cells between P _ 2 group and control group. There was no significant difference between P _ 2 group and control group. There was no significant difference between P _ 2 group and control group (P < 0.05). There was no significant difference between P _ 2 group and control group (P < 0.05). There was no significant difference between P _ 2 group and control group (P < 0.05). There was no significant difference in the area of mineralized nodule between group P1 and group P2. Conclusion the effect of hPDLCs on migration and osteogenic differentiation was significant. The results suggest that PRF has great clinical application potential in periodontal tissue regeneration engineering.
【作者单位】：郑州人民医院颐和医院口腔科;郑州市口腔医院牙周科;郑州大学口腔医学院;
【分类号】：R780.2

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