异种去抗原松质骨成骨效能的实验研究

发布时间：2018-03-20 23:32

本文选题：骨形成蛋白　切入点：异种去抗原松质骨　出处：《中国病理生理杂志》2014年08期 　论文类型：期刊论文

【摘要】：目的:研究异种去抗原松质骨与基因重组人骨形成蛋白2(recombinant human bone morphogenetic protein 2,rhBMP-2)复合后的生物相容性和成骨效能。方法:28只新西兰大白兔28处颌骨缺损完全随机分为3组:异种去抗原松质骨/rhBMP-2组(A组)、异种去抗原松质骨组(B组)和空白对照组(C组)。A组和B组每组12处骨缺损,于4、8和12周分别进行大体观察、X线检查、HE染色和新生骨量测定;C组共4处骨缺损于12周时进行大体观察、X线检查和HE染色。结果:X线显示,随着时间的增长,2组的植入骨逐渐降解吸收,由自体骨替代,A组较B组显著;C组12周时无法完成愈合。骨密度测量显示,随着时间的增长,A和B组的骨密度值增加,2组之间存在显著差异(P0.05)。HE染色可见A组的新生血管、纤维组织和新生骨均较B组多;植入骨降解速度快于B组;A组新生骨面积大于B组(P0.05)。结论:异种去抗原松质骨是rhBMP-2的良好载体;异种去抗原松质骨/rhBMP-2复合材料能持续成骨,可加速骨缺损修复的速度,是一种较好的骨修复材料。
[Abstract]:Objective: to study the biocompatibility and osteogenic potency of xenogeneic cancellous bone combined with recombinant human bone morphogenetic protein (human bone morphogenetic protein 2rhBMP-2). Methods: Twenty-eight New Zealand white rabbits were randomly divided into 3 groups. There were 12 bone defects in group A (group A), group B (group B), group C (group C) and group B (group B). Four bone defects in group C were examined with HE staining and bone mass measurement at 8 and 12 weeks, respectively. At 12 weeks, gross observation X-ray and HE staining were performed. With the increase of time, the bone grafts in group 2 gradually degraded and absorbed, and the bone graft in group A was significantly different from that in group B at 12 weeks. The bone density measurement showed that the bone mineral density (BMD) was higher than that in group C (P < 0.05). There was a significant difference in bone mineral density between group A and group B with the increase of time. The results showed that the neovascularization in group A was more than that in group B, and the number of fibrous tissue and new bone in group A was higher than that in group B. The degradation rate of implanted bone was faster than that of group B (P 0.05). Conclusion: xenogeneic cancellous bone is a good carrier of rhBMP-2, and the composite of xenogeneic cancellous bone / rhBMP-2 can sustain osteogenesis and accelerate the repair of bone defect. It is a good bone repair material.
【作者单位】：暨南大学附属第一医院口腔科;
【基金】：国家自然科学基金青年基金资助项目(No.81200930)
【分类号】：R782

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