HIF-1α对口腔癌裸鼠移植瘤生长及其CEACAM1及VEGF-C表达的影响

发布时间：2018-03-23 04:16

本文选题：口腔癌　切入点：缺氧诱导因子-α(HIF-α)　出处：《实用口腔医学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:建立口腔癌裸鼠移植瘤模型,探究HIF-1α对CEACAM1及VEGF-C表达的影响机制。方法:分别将Tca8113细胞、siRNA阴性对照Tca8113细胞、HIF-1α干扰的Tca8113细胞接种到裸鼠的右腋部皮下,建立口腔癌裸鼠移植瘤模型。观察各组裸鼠肿瘤生存情况和HIF-1α沉默对肿瘤的重量、体积抑制率的影响;采用ELISA方法检测移植瘤组织中HIF-1α干扰后其蛋白表达量的变化;分别利用实时荧光定量PCR、蛋白印迹法检测各组裸鼠移植瘤组织中HIF-1α、CEACAM1及VEGF-C mRNA和蛋白的表达水平。结果:发现HIF-1α干扰组中裸鼠移植瘤的体积及重量均显著小于空白对照组,表明HIF-1α表达沉默显著抑制移植瘤的生长,同时明显下调CEACAM1及VEGF-C的表达。结论:口腔癌中HIF-1α可能通过调节CEACAM1及VEGF-C的表达来影响肿瘤的生长。
[Abstract]:Objective: to establish a transplanted tumor model of oral carcinoma in nude mice, and to explore the mechanism of HIF-1 伪 on the expression of CEACAM1 and VEGF-C. Methods: the HIF-1 伪 interfering Tca8113 cells of Tca8113 cells were inoculated subcutaneously into the right armpit of nude mice. The tumor survival of nude mice and the effect of HIF-1 伪 silencing on tumor weight and volume inhibition rate were observed, and the expression of HIF-1 伪 protein in transplanted tumor tissue was detected by ELISA method. The expression levels of HIF-1 伪 CEACAM1, VEGF-C mRNA and protein in transplanted tumor tissues of nude mice were detected by real-time fluorescence quantitative PCR and Western blot. Results: the volume and weight of xenografts in HIF-1 伪 interference group were significantly lower than those in control group. The results indicated that HIF-1 伪 expression silencing significantly inhibited the growth of transplanted tumor, and significantly down-regulated the expression of CEACAM1 and VEGF-C. Conclusion: HIF-1 伪 may affect the growth of oral carcinoma by regulating the expression of CEACAM1 and VEGF-C.
【作者单位】：南昌大学附属口腔医院综合科·江西省口腔生物医学重点实验室;
【分类号】：R739.8

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