柠檬精油对牙周致病菌抑制作用及其安全性初步研究

发布时间：2018-03-24 20:58

本文选题：柠檬精油　切入点：牙周致病菌　出处：《口腔医学研究》2017年01期

【摘要】：目的:探讨柠檬精油对牙周致病菌的体外抗菌活性及对细胞增殖的影响。方法:采用微量液体稀释法测定柠檬精油对Pg、Fn、Aa、Pi的最小抑菌浓度(minimal inhibitory concentration,MIC)及最小杀菌浓度(minimum bactericidal concentration,MBC);以较低浓度的MIC为标准稀释LEO作为实验组,采用MTT法测定柠檬精油对HUVECs的毒性作用,明确抑菌浓度下LEO的安全性。结果:柠檬精油对牙周主要致病菌均有抑菌作用,Pg、Fn、Aa、Pi的MIC分别是9.0g/L、4.5g/L、4.5g/L、9.0g/L,Aa、Fn的MBC是9.0g/L,Pg、Pi的MBC未测得。1/2MIC、1/20MIC浓度的LEO能够抑制人脐静脉内皮细胞的生长,而低于1/200MIC浓度的LEO则对人脐静脉内皮细胞的生长没有影响,其中1/200MIC浓度的LEO作用明显优于0.02%的CHX。结论:体外环境中,柠檬精油对牙周致病菌Pg、Fn、Aa、Pi具有抗菌活性,低浓度应用对机体相对安全。
[Abstract]:Objective: to study the antibacterial activity of lemon essential oil against periodontal pathogenic bacteria in vitro and its effect on cell proliferation. Methods: the minimal inhibitory concentration and minimal bactericidal concentration of lemon essential oil to PgnfnAaPi Pi and minimal bactericidal concentration were determined by microdilution method. Bactericidal concentration was used as the standard dilution of LEO in the experimental group, and low concentration of MIC was used as the standard dilution of LEO in the experimental group. The toxicity of lemon essential oil to HUVECs was determined by MTT method. Results: the inhibitory effect of Lemon essential oil on the main periodontal pathogenic bacteria was that the MIC of LEO was 9.0g / L / L 4.5g / L 4.5g / L respectively. The MBC of 9.0g / L LEO / L LEO / FN was 9.0g / L PgCI-Pi 's MBC. The LEO at the concentration of 0.1mmMIC1m20MIC could inhibit the growth of human umbilical vein endothelial cells. LEO at lower 1/200MIC concentration had no effect on the growth of human umbilical vein endothelial cells, and the LEO effect of 1/200MIC concentration was better than that of 0.02% CHX.Conclusion: in vitro, Lemon essential oil has antibacterial activity against periodontal pathogenic bacteria PG FN AaAaAA-Pi. The application of low concentration is relatively safe for the body.
【作者单位】：广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室;天津市口腔医院;天津医科大学口腔医院口腔预防科;
【基金】：天津市卫计委科技基金(编号:2011ky16) 天津市科技发展基金面上项目(编号:10jcybjc13600)
【分类号】：R781.4

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