P53和Survivin在口腔扁平苔藓及口腔鳞状细胞癌中的表达及意义

发布时间：2018-03-26 02:10

本文选题：免疫组织化学法　切入点：口腔扁平苔藓　出处：《吉林大学》2017年硕士论文

【摘要】：研究背景:口腔扁平苔藓是一种复层鳞状上皮细胞慢性炎症性疾病,发生于黏膜和皮肤,可单独或同时发病。由于口腔扁平苔藓较正常组织具有更高的恶变倾向,WHO已经将其定义为癌前状态,其癌变率在0.4%-2%。口腔鳞状细胞癌是最常见的口腔颌面部恶性肿瘤,约占口腔所有肿瘤的90%,5年生存率只有35%-50%。口腔鳞状细胞癌具有恶性程度高、局部浸润性强、早期淋巴结转移、预后差的特征。P53是一种至关重要的抑癌基因,在食道癌、乳腺癌、膀胱癌、肝癌及宫颈癌等人类约50%的癌症中均发现了p53基因的突变。在凋亡抑制蛋白家族成员中,survivin相对分子质量最小、凋亡抑制作用最强,它与增殖、血管形成、口腔致癌作用以及口腔鳞状细胞癌相关,可能作为癌症标记物。目的:通过检测p53蛋白和survivin蛋白在口腔扁平苔藓及口腔鳞状细胞癌中的表达情况,探讨两者之间的关系以及在口腔扁平苔藓发生、发展、癌变之中的作用。方法:本实验采用免疫组织化学法检测p53蛋白、survivin蛋白在12例正常口腔黏膜组织、30例口腔扁平苔藓组织、30例口腔鳞状细胞癌组织中的表达情况,实验标本来自患者活检或手术标本取得组织。应用SPSS软件分析实验结果。结果:1.P53蛋白在正常口腔黏膜组织、口腔扁平苔藓组织和口腔鳞状细胞癌组织中的阳性表达依次增高,阳性表达率分别为16.67%(2/12)、53.33%(16/30)、80.00%(24/30),组间差别有统计学意义(p0.05)。2.Survivin蛋白在正常口腔黏膜组织、口腔扁平苔藓组织和口腔鳞状细胞癌组织中的阳性表达依次增高,阳性表达率分别为0%(0/12)、26.67%(8/30)、56.67%(17/30),组间差别有统计学意义(p0.05)。3.P53蛋白和survivin蛋白表达呈显著正相关(p0.01)。结论:1.P53蛋白在正常口腔黏膜组织、口腔扁平苔藓组织和口腔鳞状细胞癌组织中的阳性表达率依次升高,提示p53蛋白在从口腔扁平苔藓到口腔鳞状细胞癌的发展中起促进作用。2.Survivin蛋白在正常口腔黏膜组织、口腔扁平苔藓组织和口腔鳞状细胞癌组织中的阳性表达率依次升高,提示survivin蛋白在从口腔扁平苔藓到口腔鳞状细胞癌的发展中起促进作用。3.P53蛋白和survivin蛋白在口腔扁平苔藓癌变过程中的表达呈正相关,提示两者在从口腔扁平苔藓到口腔鳞状细胞癌的过程中可能起协同作用。
[Abstract]:Background: oral lichen planus (OLP) is a chronic inflammatory disease of squamous epithelium, which occurs in mucosa and skin. Oral lichen planus has a higher tendency to malignant change than normal tissue. The WHO has defined it as precancerous state, and its canceration rate is 0.4-2. Oral squamous cell carcinoma is the most common malignant tumor in oral and maxillofacial region. About 90% of all oral tumors, the 5-year survival rate is only 35% -50%. Oral squamous cell carcinoma has the characteristics of high malignancy, strong local invasion, early lymph node metastasis and poor prognosis. P53 is a crucial suppressor gene in esophageal cancer and breast cancer. P53 gene mutations were found in about 50% of human cancers such as bladder cancer, liver cancer and cervical cancer. Survivin has the least molecular weight and the strongest inhibitory effect on apoptosis in the family of apoptosis suppressor proteins, which is associated with proliferation and angiogenesis. Objective: to detect the expression of p53 protein and survivin protein in oral lichen planus and oral squamous cell carcinoma. To explore the relationship between the two and the occurrence and development of oral lichen planus, Methods: immunohistochemical method was used to detect the expression of p53 protein survivin in 30 cases of oral lichen planus and 30 cases of oral squamous cell carcinoma (OSCC). The results were analyzed by SPSS software. Results the positive expression of 1. 1. P53 protein was increased in normal oral mucosa, oral lichen planus and oral squamous cell carcinoma. The positive expression rates of survivin protein in normal oral mucosa, oral lichen planus and oral squamous cell carcinoma were significantly higher than those in normal oral mucosa, oral lichen planus and oral squamous cell carcinoma. The positive expression rates were 0 / 12 / 26.67 and 56. 67, respectively. There was a significant positive correlation between the expression of p53 protein and survivin protein in normal oral mucosa. Conclusion: 1. P53 protein is expressed in normal oral mucosa, and there is a significant positive correlation between the expression of p53 protein and survivin protein in normal oral mucosa. The positive rate of p53 protein in oral lichen planus and oral squamous cell carcinoma increased in turn, suggesting that p53 protein plays a role in promoting the development from oral lichen planus to oral squamous cell carcinoma. 2. Survivin protein in normal oral mucosa. The positive expression rates of oral lichen planus and oral squamous cell carcinoma increased in turn. The results suggest that the expression of survivin protein and survivin protein in oral lichen planus carcinogenesis is positively correlated with the development of oral lichen planus. These results suggest that both may play a synergistic role in the process from oral lichen planus to oral squamous cell carcinoma.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.5;R739.8

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