舌鳞状细胞癌组织microRNA-29b表达生物学意义研究

发布时间：2018-03-27 17:11

  本文选题：microRNA-b　切入点：舌鳞状细胞癌　出处：《中华肿瘤防治杂志》2014年17期

【摘要】：目的:探讨微小RNA-29b(microRNA-29b,miR-29b)在舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)组织中的表达及其生物学意义。方法:收集2012-01-02-2013-03-15江西省肿瘤医院头颈外科行手术切除的45例TSCC及对应癌旁组织(距离切缘2cm),qRT-PCR技术检测miR-29b在TSCC及对应癌旁组织中的表达情况,并分析其与临床病理特征的关系;应用miR-29bmimic转染人舌鳞癌Tca-8113细胞,MTT和流式细胞技术检测转染后细胞增殖和凋亡变化,qRT-PCR和蛋白质印迹法检测miR-29b潜在靶点Bcl-2mRNA及蛋白的表达变化。结果:qRT-PCR结果显示,miR-29b在TSCC组织中的表达量为0.213±0.071,显著低于对应癌旁组织的1.873±0.114,t=2.531,P=0.031;miR-29b低表达与肿瘤体积增大(≥3cm,χ2=4.022,P=0.033)及高TNM分期(Ⅲ+Ⅳ,χ2=3.398,P=0.036)具有显著相关性;MTT结果显示,在Tca-8113细胞中转染miR-29b48h后可显著降低细胞增殖能力,P0.05;同时,转染miR-29b72h后细胞凋亡率由(2.31±0.98)%增加到(11.83±1.36)%,t=2.237,P=0.034。Real-time PCR及蛋白质印迹结果显示,转染miR-29b后可显著下调Tca-8113细胞内Bcl-2mRNA及蛋白表达,P0.05。结论:TSCC组织中miR-29b表达下调并与其临床病理特征相关,miR-29b可能通过下调Bcl-2的表达来抑制舌鳞癌Tca-8113细胞增殖并促进其凋亡。
[Abstract]:Objective: to investigate the expression of microRNA-29b squamous miR-29b in tongue squamous cell carcinoma (TSCC) tissue and its biological significance. Methods: 45 cases of TSCC and corresponding adjacent tissues (distance cut) were collected from head and neck surgery of Jiangxi Provincial tumor Hospital from January 2012 to February 15, 2013 to 15, 2013. The expression of miR-29b in TSCC and adjacent tissues was detected by qRT-PCR. The relationship between it and clinicopathological features was analyzed. Cell proliferation and apoptosis were detected by miR-29bmimic transfection of human tongue squamous cell carcinoma Tca-8113 cell line and flow cytometry. MiR-29b potential target Bcl-2mRNA and protein expression were detected by qRT-PCR and Western blotting. The expression level in the tissue was 0.213 卤0.071, which was significantly lower than that in the corresponding adjacent tissues (1.873 卤0.114). The low expression of miR-29b was significantly correlated with the increase of tumor volume (鈮，

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