姜黄素对牙周炎大鼠牙周支持组织炎症性破坏的保护作用

发布时间：2018-03-29 16:37

本文选题：姜黄素　切入点：实验性牙周炎　出处：《广东医学》2017年03期

【摘要】：目的通过丝线结扎手术建立大鼠实验性牙周炎模型,观察姜黄素在大鼠实验性牙周炎中对牙周支持组织炎症性破坏的保护作用。方法选用成年雄性Sprague-Dawley大鼠30只,随机分为3组,每组10只。一组作为正常对照组,余下两组于全麻下将双侧下颌第一磨牙牙颈部进行丝线结扎,建立实验性牙周炎模型。3组均采用胃饲30 d:正常对照组给予盐水;余两组采用佐剂(2%吐温-80)(佐剂处理组)和佐剂+姜黄素(100mg/kg)(佐剂+姜黄素处理组)。最后一次给药后24 h处死所有动物。分离双侧下颌骨,一侧下颌骨去净软组织后甲基蓝着色,体视显微镜下测量釉牙骨质界到牙槽脊顶(ACJ-AC)距离用于评价骨吸收高度;另一侧下颌骨行常规组织学处理后,制备下颌第一磨牙区近远中向矢状切片,选取切片进HE染色,显微镜下观察根分叉区牙槽骨的吸收面积和骨密度。结果体视显微镜下下颌第一磨牙ACJ-AC距离测量结果显示,佐剂处理组和佐剂+姜黄素处理组ACJ-AC距离均大于正常对照组(P0.05),佐剂+姜黄素处理组ACJ-AC距离小于佐剂处理组(P0.05)。下颌第一磨牙根分叉区HE染色结果与体视显微镜下骨丧失结果相一致,佐剂处理组和佐剂+姜黄素处理组根分叉区骨丧失面积均大于正常对照组(P0.05),佐剂+姜黄素处理组根分叉区骨丧失面积小于佐剂处理组(P0.05)。结论丝线结扎手术成功造成了大鼠实验性牙周炎中牙槽骨组织的破坏,而且姜黄素的用药处理对这种牙槽骨组织的破坏起到了一定的保护作用。
[Abstract]:Objective by thread ligation operation to create a rat model of periodontitis, to observe the protective effect of curcumin in support of tissue inflammation of periodontal destruction in experimental periodontitis in rats. Methods 30 male Sprague-Dawley rats, were randomly divided into 3 groups, 10 rats in each group. One group served as normal control group, the remaining two groups under general anesthesia in the bilateral mandibular first molar tooth neck ligation, to establish experimental periodontitis model in.3 group were fed by 30 d: normal control group were given saline; the two other groups using adjuvant (2% Twain -80) (adjuvant treatment group) and adjuvant + curcumin (100mg/kg) (adjuvant + curcumin group) at last. A 24 h after the administration of animal. All animals were sacrificed bilateral mandible, mandibular to net soft tissue after methylene blue stain under stereomicroscope to measure the cemento enamel junction to the alveolar ridge (ACJ-AC) distance for evaluating bone resorption high On the other side of the mandible; for histological processing, preparation of mandibular first molar area near to the sagittal slices, the selected slice with HE staining, microscope observation of root furcation alveolar bone absorption area and bone mineral density. Results under stereomicroscope of mandibular first molar ACJ-AC distance measurement showed that the adjuvant treatment group and adjuvant + curcumin group were higher than the normal control group ACJ-AC distance (P0.05), adjuvant + curcumin group ACJ-AC adjuvant treatment group (less than P0.05). Consistent with the mandibular first molars furcation HE staining and microscope bone loss results, adjuvant treatment and adjuvant + curcumin group furcation the loss of area was larger than that of the normal control group (P0.05), adjuvant + curcumin group root furcation bone loss area is less than the adjuvant treatment group (P0.05). Conclusion the ligation operation success caused a rat experimental tooth The destruction of the alveolar bone tissue in the periodontitis and the treatment of curcumin have a certain protective effect on the destruction of the alveolar bone tissue.

【作者单位】：西南医科大学口腔医学系;西南医科大学附属口腔医院口腔预防保健科;西南医科大学附属医院口腔科;
【基金】：四川省泸州市科学技术局资助项目(编号:2013LZLY-J18)
【分类号】：R781.4

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