三种细菌在再感染根管中检出率的研究

发布时间：2018-03-31 03:37

  本文选题：白色念球菌　切入点：变形链球菌　出处：《大连医科大学》2014年硕士论文

【摘要】：目的：检测根管再治疗患者根管内白色念球菌、变形链球菌、古细菌三种细菌的检出率情况，并分析再治疗患者根管内白色念球菌、变形链球菌、古细菌三种细菌与临床症状之间的关系。 方法：临床选取131名根管根管再治疗患者89颗患牙（四川大学华西口腔医院），通过记录患牙的临床相关体征、症状等信息，去除根管内原有充填物，，插入无菌纸尖60秒，与H锉刮取物一同放入EP管中，提取基因组DNA，通过PCR反应检测白色念球菌、变形链球菌、古细菌三种细菌的16s RNA,检测根管再治疗患者根管内白色念球菌、变形链球菌、古细菌三种细菌的检出率情况，并分析再治疗患者根管内白色念球菌、变形链球菌、古细菌三种细菌与临床症状之间的关系。结果采用SPSS13.0软件进行方差分析进行统计学分析。 结果：检测根管再治疗患者根管内白色念球菌、变形链球菌、古细菌三种细菌的检出率分别为：31.46%、29.21%、19.1%。在临床症状中叩痛的病例组中的变形链球菌检出率高于无叩痛的病例组（P＜0.05）。表明变形链球菌的检出与临床症状中的叩痛密切相关，与根尖周破坏呈正相关。 结论：1、根管再治疗患者根管可以检测出白色念球菌、变形链球菌、古细菌三种细菌。 2、根管再治疗患者根管内变形链球菌的检出与临床症状中的叩痛密切相关，与根尖周破坏呈正相关。
[Abstract]:Objective: to detect the detectable rate of Candida albicans, Streptococcus mutans and Archaea in root canal retreated patients, and to analyze the detection rate of Candida albicans and Streptococcus mutans in root canals of retreated patients.The relationship between three kinds of bacteria and clinical symptoms.Methods: 89 teeth were selected from 131 patients with root canal retreatment (Huaxi Stomatological Hospital, Sichuan University). By recording the clinical signs, symptoms and other information of the affected teeth, the original filling in the root canal was removed and the sterile paper tip was inserted into the root canal for 60 seconds.The genomic DNA was extracted from EP tube with H-file scraping. The 16s RNAs of Candida albicans, Streptococcus mutans and Archaea were detected by PCR reaction, and the Candida albicans and Streptococcus mutans in root canal retreated patients were detected.The detection rate of the three kinds of bacteria was analyzed and the relationship between the clinical symptoms and the clinical symptoms of Candida albicans, Streptococcus mutans and Archaea in the root canals of retreated patients was analyzed.Results the variance analysis was carried out by SPSS13.0 software.Results: the detectable rate of Candida albicans, Streptococcus mutans and Archaea in root canal retreated patients were respectively: 1. 31.46 and 29.21. 21.The detection rate of Streptococcus mutans in patients with percussive pain was significantly higher than that in patients without percussive pain (P < 0.05).The results showed that the detection of Streptococcus mutans was closely related to the percussive pain in clinical symptoms and positively related to periapical destruction.Conclusion in the patients with root canal retherapy, Candida albicans, Streptococcus mutans and archaea can be detected in the root canal.2. The detection of Streptococcus mutans in root canal was closely related to the percussion pain in clinical symptoms and positively related to periapical destruction.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.05


本文编号：1689001