过量氟诱导成釉细胞钙超载及细胞凋亡的实验研究

发布时间：2018-03-31 11:50

本文选题：氟　切入点：成釉细胞　出处：《华西口腔医学杂志》2014年06期

【摘要】：目的研究过量氟对体外培养大鼠成釉细胞内钙超载及细胞凋亡的影响。方法取大鼠成釉细胞系HAT-7细胞,分别加入不同浓度(0、0.4、0.8、1.6、3.2、6.4 mmol·L-1)的氟化钠培养液,培养48 h后,采用Cell Counting Kit 8(CCK-8)试剂盒检测各组细胞的活性,流式细胞术分析氟对细胞凋亡的影响,激光扫描共聚焦显微镜、Western blot试验和实时荧光定量聚合酶链反应技术检测过量氟诱导大鼠成釉细胞内Ca2+浓度和钙网蛋白表达的变化。结果氟化钠浓度高于1.6 mmol·L-1时,可抑制成釉细胞的活性,成釉细胞内Ca2+浓度升高,钙网蛋白表达上调,细胞早期凋亡数量增加,并且随着浓度的增加,细胞凋亡的数量也随之增加。结论过量氟可引起成釉细胞内钙超载,诱导成釉细胞凋亡。
[Abstract]:Objective to study the effect of excess fluoride on calcium overload and apoptosis of ameloblast in vitro.Methods Rat ameloblast cell line HAT-7 cells were cultured in different concentration of sodium fluoride culture medium (0.40.40.40.80.80.63.2ng 6.4 mmol L -1) for 48 h. The activity of each group was detected by Cell Counting Kit 8CCK-8 kit. The effect of fluoride on apoptosis was analyzed by flow cytometry (FCM), and the effect of fluoride on cell apoptosis was analyzed by flow cytometry (FCM), and the effect of fluoride on cell apoptosis was analyzed by flow cytometry (FCM).Laser scanning confocal microscopy Western blot assay and real-time fluorescence quantitative polymerase chain reaction were used to detect the changes of Ca2 concentration and calcium reticulin expression in rat ameloblasts induced by excessive fluoride.Results when the concentration of sodium fluoride was higher than 1.6 mmol L-1, the activity of ameloblast was inhibited. The concentration of Ca2 in ameloblast was increased, the expression of calmodulin was up-regulated, and the number of early apoptosis was increased with the increase of concentration.The number of apoptosis also increased.Conclusion excessive fluoride can induce calcium overload in ameloblasts and induce apoptosis of ameloblasts.
【作者单位】：中国医科大学口腔医学院口腔预防教研室;辽宁省口腔医学研究所;中国医科大学口腔医学院中心实验室;
【基金】：国家自然科学基金资助项目(81072245) 辽宁省自然科学基金资助项目(20102278)
【分类号】：R780.2

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