机械加载促进骨质疏松大鼠种植体骨结合的实验研究

发布时间：2018-04-01 22:14

本文选题：机械加载　切入点：骨质疏松　出处：《天津医科大学学报》2017年05期

【摘要】：目的:利用骨质疏松实验动物模型,探讨机械加载促进种植体周围骨结合的效能。方法:12只SD雌性大鼠(12周龄)卵巢切除建立绝经后骨质疏松动物模型(OVX),另12只动物设为对照。4周后所有动物均于双侧股骨中段植入直径1.5 mm,长度4.5 mm的纯钛种植体。按术式分别随机分组如下:假手术组(sham)、假手术膝关节加载治疗组(sham+loading)、OVX模型组(OVX)和OVX膝关节加载治疗组(OVX+loading)(n=6)。种植术后第2天对双侧膝关节进行机械加载刺激(5 N,10 Hz,3 min/侧,共6 min/d,加载5 d,休息2 d,往复循环4周)。通过卵巢切除前和机械加载后测量的骨密度(BMD)和骨矿化度(BMC)、生物力学测试对治疗效果进行评估。结果:Sham+loading组与sham组相比,膝关节机械加载显著改善BMD和BMC(P0.001)。OVX+loading组与OVX对照组相比,膝关节机械加载明显增加BMD和BMC(P0.001)。生物力学测试表明,Sham+loading组与sham组相比,膝关节机械加载显著改善种植体最大推出力(P0.001)。OVX+loading组与OVX对照组相比,膝关节机械加载明显增加种植体最大推出力(P0.001)。结论:机械加载显著改善了骨质疏松动物的骨密度,促进骨质疏松大鼠种植体周围的骨结合。该研究对口腔临床种植修复开辟了新思路。
[Abstract]:Objective: to study the effect of mechanical loading on bone binding around implants by using animal model of osteoporosis.Methods OVX, a postmenopausal osteoporosis animal model, was established by ovariectomy in 12 SD female rats. After 4 weeks of control, all the animals were implanted with titanium implants of 1.5 mm in diameter and 4.5 mm in length in the middle femur of both sides.According to the operation methods, they were randomly divided as follows: Shammao in sham operation group, OVXX in sham loading treatment group and OVXX in OVX knee joint loading treatment group (OVX loading treatment group) and OVX loading treatment group (OVX loading treatment group) and OVX knee joint loading treatment group (OVX loading treatment group).On the second day after implantation, the bilateral knee joints were subjected to mechanical stimulation of 5 NV 10 Hz / 3 min/ for 6 min / d for 5 days, rest for 2 days, and reciprocating for 4 weeks.Bone mineral density (BMD) and bone mineralization (BMC) were measured before and after ovariectomy and mechanical loading. Biomechanical tests were performed to evaluate the therapeutic effect.Results compared with the sham group, the mechanical loading of knee joint in the BMD group and the BMC(P0.001).OVX loading group was significantly improved compared with that in the OVX control group, and the mechanical loading of the knee joint increased the BMD and BMC P 0.001 significantly compared with the control group.Biomechanical test showed that mechanical loading of knee joint in Sham loading group significantly improved the maximal protruding force of implant P0.001. OVX loading group compared with OVX control group. Mechanical loading of knee joint significantly increased the maximum protruding force of implant P0.001.Conclusion: mechanical loading can significantly improve bone mineral density and promote bone bonding around implants in osteoporosis rats.This study has opened up a new idea for dental implant repair.
【作者单位】：天津医科大学人体解剖与组织胚胎学系;天津医科大学口腔医学院口腔颌面外科;
【基金】：国家自然科学基金资助项目(81572100,81601863) 天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36500)
【分类号】：R783.6

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