CEACAM1表达和舌癌瘤内及瘤周淋巴管生成密度关系的观察研究

发布时间：2018-04-02 13:39

本文选题：舌鳞癌　切入点：瘤内淋巴管　出处：《天津医科大学》2014年硕士论文

【摘要】：研究目的：1、以淋巴管特异性标记物癌胚抗原相关细胞粘附因子1(CEACAM1)标记人舌鳞癌组织中淋巴管,并计算淋巴管密度(LMVD)值,观察CEACAM1在人舌鳞癌瘤内及瘤周淋巴管中的表达情况,探讨CEACAM1及其受体在舌癌发生、发展和淋巴转移中的作用及意义。 2、将舌鳞癌细胞株正位植入裸鼠左侧舌缘构建舌鳞癌动物模型,从而模拟舌体内环境,观察舌癌细胞的生物学行为及舌鳞癌模型的淋巴结转移情况,采用免疫组织化学染色的方法标记舌鳞癌病灶及转移淋巴结中的淋巴管,并观察肿瘤淋巴管的情况及其与舌鳞癌淋巴道转移的相关性。研究方法：1、收集72例舌鳞癌病灶及转移淋巴结的病理标本,其中43例(男性24例,女性19例)人舌鳞癌颈淋巴结转移阳性患者的组织和29例(男性17例,女性12例)人舌鳞癌颈淋巴结转移阴性患者的组织,采用免疫组织化学染色方法检测病理组织中的表达,用兔抗人CEACAM1标记淋巴管,并计数人舌鳞癌瘤内与瘤周组织淋巴管生成的密度,并进行统计学分析。2、将人舌鳞癌Tca83细胞悬液正位移植于裸鼠左侧舌缘,隔日观察并记录裸鼠体重、舌体成瘤情况、病灶的大小,待裸鼠自然死亡或体质量下降至原体质量的25%时,切取舌部病灶及颈部淋巴结,进行HE染色和免疫组化检测舌鳞癌组织的CEACAM1表达,用兔抗人CEACAM1标记淋巴管,并计数人舌鳞癌瘤内与瘤周组织淋巴管生成的密度,并进行统计学分析。研究结果：1、人舌鳞癌病理标本的癌灶内可见散在的、孤立的新生不完整管状结构,未与周围宿主淋巴管相连通,这些管状结构内可见较多的内皮细胞间隙,管腔细窄；瘤周可见淋巴管数量增多、管腔扩张、开放。通过淋巴管计数,瘤周淋巴管密度(16.748±3.070)高于瘤内淋巴管密度(8.662±3.214),差异有显著性(P0.01)。 2、裸鼠在接种后第6-12天相继出现舌部肿瘤,成瘤率为77.14%,通过HE染色及免疫组化染色检测的淋巴结转移阳性率为34.29%,镜下可见舌鳞癌瘤内和瘤周内都有阳性表达,表现为淋巴管形态不规则,管壁薄,可呈开放状态,腔内不含红细胞,与血管可明显区别,有时有胶冻状淋巴液；也可表现为管腔塌陷、闭锁呈条索状,甚至可见单个内皮细胞围成管腔。通过淋巴管计数,瘤周淋巴管密度(5.488-2.114)高于瘤内淋巴管密度(3.026±1.470),差异具有显著性(P0.01)。研究结论：1、(1) CEACAMl对淋巴管内皮细胞具有很强的特异性与敏感性,可广泛用于淋巴管的研究。(2)舌鳞癌的瘤内和瘤周组织中,特别是瘤周组织存在淋巴管生成,增加了肿瘤细胞淋巴转移的机会,在舌鳞癌的生长和转移过程中发挥了重要作用。 2、成功建立裸鼠舌鳞癌正位模型,为研究在体内环境中舌癌的生长、转移机制提供了重要的实验手段。通过比较裸鼠舌鳞癌瘤内淋巴管密度与瘤周淋巴管密度,推断淋巴管的生成可能对舌鳞癌淋巴道转移有一定促进作用。
[Abstract]:Objective to investigate the expression of CEACAM1 in human tongue squamous cell carcinoma (LMVD), and to investigate the expression of CEACAM1 in human tongue squamous cell carcinoma (LMVD), using lymphangio-specific marker carcinoembryonic antigen associated cell adhesion factor 1 CEACAM1, and to calculate the density of lymphatic vessels in human tongue squamous cell carcinoma (LMVD), and to observe the expression of CEACAM1 in human tongue squamous cell carcinoma and its surrounding lymphatic vessels. To investigate the role and significance of CEACAM1 and its receptors in the development, development and lymphatic metastasis of tongue cancer. 2. The tongue squamous cell carcinoma cell line was implanted into the left tongue margin of nude mice to construct the animal model of tongue squamous cell carcinoma, and the biological behavior of tongue cancer cell line and lymph node metastasis of tongue squamous cell carcinoma model were observed. The lymphatic vessels in squamous cell carcinoma of tongue and metastatic lymph nodes were labeled by immunohistochemical staining, and the correlation between lymphatic vessels and lymphatic metastasis in squamous cell carcinoma of tongue was observed. Methods 72 specimens of squamous cell carcinoma of tongue and metastatic lymph nodes were collected, including 43 cases (24 males, 19 females) and 29 cases (17 males) of cervical lymph node metastasis of tongue squamous cell carcinoma. The expression of lymphatic vessels was detected by immunohistochemical staining in 12 cases of human tongue squamous cell carcinoma with negative cervical lymph node metastasis. Rabbit anti-human CEACAM1 was used to label lymphatic vessels. The density of lymphangiogenesis in and around human tongue squamous cell carcinoma was counted, and the density of lymphangiogenesis was analyzed statistically. The Tca83 cell suspension of human tongue squamous cell carcinoma was transplanted into the left lingual margin of nude mice. The body weight and the tumorigenesis of tongue were observed and recorded every other day. When the size of the lesion was reduced to 25% of the mycoplasma mass in nude mice, the tongue lesion and cervical lymph nodes were removed, and the expression of CEACAM1 in tongue squamous cell carcinoma was detected by HE staining and immunohistochemistry. The lymphatic vessels were labeled with rabbit anti-human CEACAM1. The density of lymphatic vessels in and around human tongue squamous cell carcinoma was counted and analyzed statistically. Results: 1. In the pathological specimens of human tongue squamous cell carcinoma, scattered and isolated new incomplete tubular structures were found, which were not connected with the lymphatic vessels of the surrounding host. There were more endothelial cell interspaces and narrow lumen in these tubular structures. The number of lymphatic vessels increased, the lumen dilated and opened around the tumor, and the density of lymphatic vessels around the tumor was 16.748 卤3.070), which was higher than that of the intratumoral lymphatic vessels (8.662 卤3.214), and the difference was significant (P 0.01). 2. Tongue tumors appeared in nude mice 6-12 days after inoculation, the tumorigenesis rate was 77.14 and the positive rate of lymph node metastasis detected by HE staining and immunohistochemical staining was 34.29. The lymphatic vessels are characterized by irregular morphology, thin wall, open state, no red blood cells in the lumen, which can be distinguished from the blood vessels, sometimes with gelatinous lymphatic fluid; they can also be shown as cavities collapsing, atresia being striped, The density of lymphatic vessels around the tumor (5.488-2.114) was higher than that of the intratumoral lymphatic vessels (3.026 卤1.470), and the difference was significant (P 0.01). Conclusion CEACAMl has strong specificity and sensitivity to lymphatic endothelial cells, and can be widely used in the study of lymphatic vessels. 2) lymphangiogenesis exists in intratumor and perineoplastic tissues of tongue squamous cell carcinoma, especially in surrounding tissues. It increases the chance of lymphatic metastasis of tumor cells and plays an important role in the growth and metastasis of tongue squamous cell carcinoma. 2. The positive position model of tongue squamous cell carcinoma in nude mice was successfully established, which provided an important experimental means for studying the growth and metastasis mechanism of tongue carcinoma in vivo. It is concluded that lymphangiogenesis may promote lymphatic metastasis in squamous cell carcinoma of tongue.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.86

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